|
Licensing opportunities: basic fluorescent proteins
- Multicolor protein collection for in vivo labeling applications
- Direct expression in cells
- For research and commercial use
Evrogen offers proprietary collection of fluorescent proteins optimized for various applications in commercial biotechnology, cell biology and biomedicine.
Diversity of Evrogen basic fluorescent proteins
Ranging in color from blue to far-red, Evrogen fluorescent proteins can be used for multicolor labeling to observe different cellular events in a particular cell or a cell population.
|
|
Normalized excitation / emission spectra
Enlarge
View in the Spectra viewer tool
|
List of fluorescent proteins
| PRODUCT PAGE |
COLOR |
EX/EM MAX (nm) |
BRIGHTNESS,
% OF EGFP |
STRUCTURE |
MATURATION RATE AT 37°C |
DESCRIPTION (PDF) |
* Purified recombinant protein behaves as a pure monomer at concentrations of 10 mg/ml as verified by high-performance liquid chromatography (HPLC).
** Although NirFP is characterized by fast maturation in E. coli, due to low brightness it requires up to 48 hours for detection after transfection in mammalian cells.
|
| TagFPs, protein localization tags / details  |
|
| TagBFP | blue | 402 / 457 | 99 | monomer | fast |  |
| TagCFP | cyan | 458 / 480 | 64 | monomer | fast | |
| TagGFP2 | green | 483 / 506 | 105 | monomer | fast | |
| TagYFP | yellow | 508 / 524 | 94 | monomer | fast | |
| TagRFP | red (orange) | 555 / 584 | 148 | monomer | fast | |
| FusionRed | red | 580 / 608 | 53 | supermonomer* | fast | |
| mKate2 | far-red | 588 / 633 | 74 | monomer | fast | |
| TurboFPs, superbright and fast-maturing proteins / details |
|
| TurboGFP | green | 482 / 502 | 112 | dimer | super fast | |
| TurboYFP | yellow | 525 / 538 | 169 | dimer | super fast | |
| TurboRFP | red (orange) | 553 / 574 | 187 | dimer | super fast | |
| TurboFP602 | true-red | 574 / 602 | 79 | dimer | fast | |
| TurboFP635 | far-red | 588 / 635 | 67 | dimer | super fast | |
| Fluorescent proteins for whole body imaging / details |
|
| Katushka2S | far-red | 588 / 633 | 89 | dimer | super fast | |
| TurboFP650 | near-infrared | 592 / 650 | 47 | dimer | super fast | |
| NirFP | near-infrared | 605 / 670 | 13 | dimer | fast** | |
| Fluorescent proteins for fluorescence lifetime imaging microscopy (FLIM) / details |
|
| BrUSLEE | green | 487 / 509 | 78 | | fast | |
| SLIM | green | 488 / 508 | 13 | | fast | |
| Old or discontinued variants |
|
| TagGFP | green | 482 / 505 | 104 | monomer | fast | |
| TagFP635 | far-red | 588 / 635 | 45 | monomer | fast | |
| JRed | true-red | 584 / 610 | 26 | weak dimer | slow | |
| PhiYFP | yellow | 525 / 537 | 158 | dimer | fast | |
| CopGFP | green | 482 / 502 | 126 | tetramer | super fast | |
Evaluation of the technologies is easily available by purchase of the related research use product. Please see "Notice to Purchaser" section for the product of your interest for details.
For license information please contact Evrogen by e-mail at license@evrogen.com.
References:
-
Chudakov DM, Lukyanov S, Lukyanov KA.
Fluorescent proteins as a toolkit for in vivo imaging.
Trends Biotechnol. 2005 Dec;23(12):605-13. / pmid: 16269193
-
Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM.
Structural basis for the fast maturation of Arthropoda green fluorescent protein.
EMBO Rep. 2006; 7 (10):1006-12. / pmid: 16936637
-
Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.
Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
-
Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
-
Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
Bright far-red fluorescent protein for whole-body imaging.
Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
|
