|
Green fluorescent protein TagGFP2
- Bright green fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and FRET applications
 |
TagGFP2 (scientific name mTagGFP) is the improved variant of TagGFP, the mutant of the Aequorea macrodactyla GFP-like protein [Xia et al., 2002, Subach et al., 2008]. TagGFP2 possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively.
TagGFP2 matures 1.6-fold faster than TagGFP and is characterized by the improved performance in fusions. Compared to EGFP, TagGFP2 provides about the same brightness of fluorescence but is significantly more pH stable. TagGFP2 is specially optimized for expression at 37°C.
Because of monomeric nature, TagGFP2 is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, TagGFP2 can be used as a donor for red fluorescent protein TagRFP or as an acceptor for blue fluorescent protein TagBFP.
|
Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
|
| TagGFP2 expression/source vectors |
|
|
|---|
| pTagGFP2-C |
FP191 |
Mammalian expression vector encoding humanized TagGFP2 and allowing its expression and generation of fusions to the TagGFP2 C-terminus |
20 μg |
€ 400 / 200* |
| pTagGFP2-N |
FP192 |
Mammalian expression vector encoding humanized TagGFP2 and allowing its expression and generation of fusions to the TagGFP2 N-terminus |
20 μg |
€ 400 / 200* |
| pTagGFP2-actin |
FP194 |
Mammalian expression vector encoding humanized TagGFP2 fused with human cytoplasmic β-actin |
20 μg |
€ 400 / 200* |
| pTagGFP2-tubulin |
FP195 |
Mammalian expression vector encoding humanized TagGFP2 fused with human α-tubulin |
20 μg |
€ 400 / 200* |
| pTagGFP2-mito |
FP197 |
Mammalian expression vector encoding humanized TagGFP2 targeted to mitochondria |
20 μg |
€ 400 / 200* |
|
| Recombinant protein |
|
|
|---|
| rTagGFP2 |
FP152 |
Purified recombinant monomeric green fluorescent protein |
100 μg |
€ 130 |
|
| Antibody against TagGFP2 |
|
|
|---|
Anti-GFP
antibody |
AB011 |
Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer |
100 μg |
€ 220 |
Anti-Tag(CGY)FP
antibody |
AB121 |
Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM |
100 μg |
€ 220 |
References:
-
Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV.
Conversion of Red Fluorescent Protein into a Bright Blue Probe.
Chem Biol. 2008; 15 (10):1116-24. doi: 10.1016/j.chembiol.2008.08.006 / pmid: 18940671
-
Xia NS, Luo WX, Zhang J, Xie XY, Yang HJ, Li SW, Chen M, Ng MH.
Bioluminescence of Aequorea macrodactyla, a common jellyfish species in the East China Sea.
Mar Biotechnol (NY). 2002; 4 (2):155-62. / pmid: 14961275
|
