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    SLIM

SLIM
GFP with short fluorescence lifetime

- Green fluorescence
- Short fluorescence lifetime (1300 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)
- Fast maturation and high photostability

SLIM (Shortened Lifetime Imaging Marker) is a highly photostable green fluorescent protein with fluorescence lifetime shortened/reduced relative to the common GFPs (1300 ps vs 2.5-3 ns) [Mamontova et al., 2018]. SLIM carries glycine-tyrosine-glycine (GYG) chromophore and demonstrates fast chromophore maturation under physiological conditions. It also shows weak reactivity in excited-state redox-chemistry and thus – low phototoxicity.

For multiparameter FLIM in green channel SLIM can be used with fluorescent proteins with similar fluorescence spectra and different fluorescence lifetimes: BrUSLEE (800 ps), SLIM (1.3 ns), TagGFP2 (3.2 ns).

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SLIM expression/source vector

pSLIM-N FP212 Mammalian expression vector encoding humanized SLIM and allowing its expression and generation of fusions to the SLIM N-terminus 20 μg € 400


Antibody against SLIM

Anti-GFP
antibody
AB011 Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer 100 μg € 220
Anti-Tag(CGY)FP
antibody
AB121 Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM 100 μg € 220

References:

  • Mamontova AV, Solovyev ID, Savitsky AP, Shakhov AM, Lukyanov KA, Bogdanov AM. Bright GFP with subnanosecond fluorescence lifetime. Sci Rep. 2018; 8 (1):13224. doi: 10.1038/s41598-018-31687-w. / pmid: 30185895
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