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GFP with subnanosecond fluorescence lifetime

- Bright green fluorescence
- Fast maturation and enhanced photostability
- Short fluorescence lifetime (800 ps, in vitro)
- Recommended for fluorescence lifetime imaging microscopy (FLIM)

BrUSLEE (Bright Ultimately Short Lifetime Enhanced Emitter) possesses short fluorescence lifetime (800 ps) and high brightness (78% of EGFP) [Mamontova et al., 2018]

Compared to other fluorescent proteins with the shortest fluorescence lifetimes reported to date mGarnet2 (760 ps) and TagRFP675 (900 ps), BrUSLEE has exceptional fluorescence brightness (QY = 0.3, EC = 86 000 M−1cm−1)

Because of this unique combination f brightness, photostability and subnanosecond fluorescence lifetime, BrUSLEE is mainly intended for fluorescence lifetime imaging microscopy.

For multiparameter FLIM in green channel BrUSLEE can be used with fluorescent proteins with similar fluorescence spectra and different fluorescence lifetimes: BrUSLEE (800 ps), SLIM (1.3 ns), TagGFP2 (3.2 ns).

Related products
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BrUSLEE expression/source vector

pBrUSLEE-N FP214 Mammalian expression vector encoding humanized BrUSLEE and allowing its expression and generation of fusions to the BrUSLEE N-terminus 20 μg € 400

Antibody against BrUSLEE

AB011 Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer 100 μg € 220
AB121 Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM 100 μg € 220


  • Mamontova AV, Solovyev ID, Savitsky AP, Shakhov AM, Lukyanov KA, Bogdanov AM. Bright GFP with subnanosecond fluorescence lifetime. Sci Rep. 2018; 8 (1):13224. doi: 10.1038/s41598-018-31687-w. / pmid: 30185895
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