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Blue fluorescent protein TagBFP
- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications
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TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
Compared to EBFP2 [Ai et al., 2007], TagBFP is more then 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of TagBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.
Good overlap between the emission spectrum of TagBFP and the absorbance spectra of TagGFP2 allows using these two proteins as a FRET pair.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| TagBFP expression/source vectors |
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| pTagBFP-C |
FP171 |
Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP C-terminus |
20 μg |
€ 400 / 200* |
| pTagBFP-N |
FP172 |
Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP N-terminus |
20 μg |
€ 400 / 200* |
| pTagBFP-H2B |
FP176 |
Mammalian expression vector encoding humanized TagBFP fused with human histone H2B |
20 μg |
€ 400 / 200* |
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| Antibody against TagBFP |
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Anti-tRFP
antibody |
AB233 |
Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry |
100 μg |
€ 220 |
References:
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Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE.
Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins.
Biochemistry. 2007; 46 (20):5904-10. / pmid: 17444659
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Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV.
Conversion of Red Fluorescent Protein into a Bright Blue Probe.
Chem Biol. 2008; 15 (10):1116-24. doi: 10.1016/j.chembiol.2008.08.006 / pmid: 18940671
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