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RESEARCH PRODUCTS/Basic fluorescent proteins/TurboFPs:

    TurboFP635

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Far-red fluorescent protein TurboFP635

- Super bright far-red fluorescence
- Fast maturation, high photostability
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for cell and organelle labeling in autofluorescent environment, multicolor applications and whole body imaging

TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor [Shcherbo et al., 2007]. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close HcRed [Gurskaya et al., 2001] or mPlum [Wang et al., 2004]. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays.

TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for multicolor imaging, tracking the promoter activity in auto-fluorescent tissues, cell and organelle labeling.

detailed description

 
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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


TurboFP635 expression/source vectors

pTurboFP635-C FP721 Mammalian expression vector encoding humanized TurboFP635 and allowing its expression and generation of fusions to the TurboFP635 C-terminus 20 μg € 320 / 160*
pTurboFP635-N FP722 Mammalian expression vector encoding humanized TurboFP635 and allowing its expression and generation of fusions to the TurboFP635 N-terminus 20 μg € 320 / 160*


Recombinant protein

rTurboFP635 FP752 Purified recombinant bright red fluorescent protein 100 μg € 130


Antibody against TurboFP635

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry 100 μg € 220

References:

  • Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA. GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 2001; 507 (1):16-20. / pmid: 11682051
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
  • Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004; 101 (48):16745-9. / pmid: 15556995
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