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Red fluorescent protein TagRFP
- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications
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TagRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. TagRFP is about three times brighter than mCherry protein [Shaner et al., 2004], which makes it the brightest monomeric red fluorescent protein available so far.
TagRFP is mainly intended for protein labeling and FRET applications. It can also be used for cell and organelle labeling and for tracking the promoter activity.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| TagRFP expression/source vectors |
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| pTagRFP-C |
FP141 |
Mammalian expression vector encoding humanized TagRFP and allowing its expression and generation of fusions to the TagRFP C-terminus |
20 μg |
€ 400 / 200* |
| pTagRFP-N |
FP142 |
Mammalian expression vector encoding humanized TagRFP and allowing its expression and generation of fusions to the TagRFP N-terminus |
20 μg |
€ 400 / 200* |
| pTagRFP-tubulin |
FP145 |
Mammalian expression vector encoding humanized TagRFP fused with human α-tubulin |
20 μg |
€ 400 / 200* |
| pTagRFP-mito |
FP147 |
Mammalian expression vector encoding humanized TagRFP targeted to mitochondria |
20 μg |
€ 400 / 200* |
| Gateway® TagRFP-AS-C entry clone |
FP148 |
Gateway® entry clone for generation of fusions to the C-terminus of TagRFP; transfer of the constract encoding TagRFP or its fusion into Gateway® destination vectors; TagRFP codon usage is optimized for expression in Arabidopsis and Saccharomyces |
20 μg |
€ 400 / 200* |
| Gateway® TagRFP-AS-N entry clone |
FP149 |
Gateway® entry clone for generation of fusions to the N-terminus of TagRFP; transfer of the constract encoding TagRFP or its fusion into Gateway® destination vectors; TagRFP codon usage is optimized for expression in Arabidopsis and Saccharomyces |
20 μg |
€ 400 / 200* |
| pTagRFP-Cx43 |
FP364 |
Mammalian expression vector encoding humanized TagRFP fused with rat connexin 43 |
20 μg |
€ 400 / 200* |
| pTagRFP-Golgi |
FP367 |
Mammalian expression vector encoding humanized TagRFP fused with human Golgi targeting sequence (GTS) |
20 μg |
€ 400 / 200* |
| pTagRFP-H2B |
FP368 |
Mammalian expression vector encoding humanized TagRFP fused with human histone H2B |
20 μg |
€ 400 / 200* |
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| Recombinant protein |
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| rTagRFP |
FP154 |
Purified recombinant monomeric red (orange) fluorescent protein |
100 μg |
€ 130 |
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| Antibody against TagRFP |
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Anti-tRFP
antibody |
AB233 |
Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry |
100 μg |
€ 220 |
References:
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Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.
Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
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Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY.
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
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Shcherbo D, Souslova EA, Goedhart J, Chepurnykh TV, Gaintzeva A, Shemiakina II, Gadella TW, Lukyanov S, Chudakov DM.
Practical and reliable FRET/FLIM pair of fluorescent proteins.
BMC Biotechnol. 2009; 9 :24. doi: 10.1186/1472-6750-9-24 / pmid: 19321010
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