TagFPs: protein localization tags

- Bright monomeric proteins; excellent performance in fusions
- Wide color palette
- For research and commercial use

TagFPs group comprises proteins that are optimized for protein localization / interaction studies and stable expression in long term cultures. They can be also used for other cell labeling applications.
TagFPs demonstrate successful performance in fusions with cellular proteins and can be expressed in various heterological systems. Ranging in color from blue to far-red, they provide unique possibilities for multicolor labeling of subcellular structures.

FRET pairs

ready-to-use vectors for protein labeling

Spectral diversity

Normalized excitation / emission spectra

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Available proteins

CHARACTERISTIC	TagBFP	TagCFP	TagGFP2	TagYFP	TagRFP	FusionRed	mKate2
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000. ** Purified recombinant protein behaves as a pure monomer at concentrations of 10 mg/ml as verified by high-performance liquid chromatography (HPLC). Ext.coeff. – extinction coefficient.


Fluorescence color	blue	cyan	green	yellow	red (orange)	red	far-red
Excitation max (nm)	402	458	483	508	555	580	588
Emission max (nm)	457	480	506	524	584	608	633
Quantum yield	0.63	0.57	0.6	0.62	0.48	0.19	0.40
Ext. coeff. (M^-1cm^-1)	52 000	37 000	56 500	50 000	100 000	94 500	62 500
Brightness*	32.8	21.1	33.9	31	48.0	18.0	25.0
Brightness, % of EGFP	99	64	105	94	148	53	74
pKa	2.7	4.7	5.0	5.5	3.8	4.6	5.4
Structure	monomer	monomer	monomer	monomer	monomer	supermonomer**	monomer
Cell toxicity	not observed	not observed	not observed	not observed	not observed	not observed	not observed
Aggregation	no	no	no	no	no	no	no
Maturation rate at 37°C	fast	fast	fast	fast	fast	fast	fast
Molecular weight (kDa)	26	27	27	27	27	26	26


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