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Red fluorescent protein FusionRed
- Superior performance in fusions
- Low cytotoxicity
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and long-term experiments
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FusionRed is a red fluorescent protein characterized by improved performance in fusions and low toxicity [Shemiakina et al., 2012]. FusionRed lacks the residual tendency of other monomeric RFPs to dimerize at high concentration and behaves as a pure monomer at concentrations up to 10 mg/ml in HPLC analysis. Such "supermonomeric" properties ensure superior efficiency of FusionRed in protein labeling applications, especially in the cells with high expression level.
Similarly to parental mKate2, FusionRed demonstrates fast maturation rate, high pH-stability and photostability, and significantly lower cytotoxicity than widely used mCherry [Shaner et al., 2004] and mRuby [Kredel et al., 2009].
FusionRed is mainly intended for protein labeling and long-term experiments including generation of transgenic animals.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| FusionRed expression/source vectors |
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| pFusionRed-N |
FP412 |
Mammalian expression vector encoding humanized FusionRed and allowing its expression and generation of fusions to the FusionRed N-terminus |
20 μg |
€ 480 / 240* |
| pFusionRed-Cx43 |
FP417 |
Mammalian expression vector encoding humanized FusionRed fused with rat connexin 43 |
20 μg |
€ 480 / 240* |
| pFusionRed-f-mem |
FP418 |
Mammalian expression vector encoding membrane-targeted FusionRed |
20 μg |
€ 480 / 240* |
| pFusionRed-H2B |
FP421 |
Mammalian expression vector encoding humanized FusionRed fused with human histone H2B |
20 μg |
€ 480 / 240* |
| pFusionRed-endo |
FP427 |
Mammalian expression vector encoding humanized FusionRed fused with human RhoB protein |
20 μg |
€ 480 / 240* |
| pFusionRed-PDHA1 |
FP430 |
Mammalian expression vector encoding humanized FusionRed fused with human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1) |
20 μg |
€ 480 / 240* |
| pFusionRed-Rab5a |
FP431 |
Mammalian expression vector encoding humanized FusionRed fused with human Ras-related protein Rab-5A |
20 μg |
€ 480 / 240* |
| pFusionRed-talin |
FP432 |
Mammalian expression vector encoding humanized FusionRed fused with mouse talin-1 protein |
20 μg |
€ 480 / 240* |
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| Antibody against FusionRed |
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Anti-tRFP
antibody |
AB233 |
Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry |
100 μg |
€ 220 |
References:
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Kredel S, Oswald F, Nienhaus K, Deuschle K, Röcker C, Wolff M, Heilker R, Nienhaus GU, Wiedenmann J.
mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures.
PLoS One. 2009; 4 (2):e4391. doi: 10.1371/journal.pone.0004391 / pmid: 19194514
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Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY.
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
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Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D.
A monomeric red fluorescent protein with low cytotoxicity.
Nat Commun. 2012; 3 :1204. doi: 10.1038/ncomms2208 / pmid: 23149748
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