Tools for labeling of subcellular structures

- Easy labeling of cell compartments and proteins
- Wide range of fluorescent colors
- No exogenous chemical compounds or cofactors required

Ready-to-use expression vectors are available for labeling of:

Organelles	Cytoskeletal and adhesion proteins	Gap junction proteins	Nuclear proteins
endoplasmic reticulum endosomes Golgi apparatus lysosomes membrane mitochondria peroxisomes	β-actin α-actinin VE-cadherin EB3 protein microtubules talin α-tubulin	connexin 43 Vesicular transport proteins clathrin LCB	histone H2B lamin B1 Signal transduction proteins annexin CD151 CDC42 paxillin Rab-5A Metabolic pathway proteins PDHA1

Evrogen collection of fluorescent proteins provides wide opportunities for subcellular imaging. In addition to ready-to-use subcellular localization vectors, vectors comprising fluorescent protein coding sequence linked with multiple cloning sites allow easy targeting of the reporter in the specific cellular compartment. The targeting signal can be fused to C- or N- end of the fluorescent protein using appropriate expression vector (marked with "-C" or "-N" correspondingly). Unmodified vectors can be used for cytosolic expression of fluorescent markers.

Fluorescent microscopy of mammalian cells expressing Evrogen fluorescent proteins targeted to the different subcellular structures and proteins