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References citing TagBFP:

  • Rowland DC, Weible AP, Wickersham IR, Wu H, Mayford M, Witter MP, Kentros CG.
    Transgenically targeted rabies virus demonstrates a major monosynaptic projection from hippocampal area CA2 to medial entorhinal layer II neurons.
    J Neurosci. 2013 Sep 11;33(37):14889-98 doi: 10.1523/JNEUROSCI.1046-13.2013
    pmid: 24027288
  • Lee S, Lim WA, Thorn KS.
    Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae.
    PLoS One. 2013 Jul 2;8(7):e67902 doi: 10.1371/journal.pone.0067902
    pmid: 23844123
  • Gaur NA, Hasek J, Brickner DG, Qiu H, Zhang F, Wong CM, Malcova I, Vasicova P, Brickner JH, Hinnebusch AG.
    Vps factors are required for efficient transcription elongation in budding yeast.
    Genetics. 2013 Mar;193(3):829-51 doi: 10.1534/genetics.112.146308
    pmid: 23335340
  1. BFP was fused to a yeast nuclear pore protein, anti-tRFP antibodies were used in Western blot analysis.
  2. Constructs used: TagBFP fused to NSP1.
  3. Expression system: The yeasts.
  4. Detection system: Olympus IX-81 inverted microscope equipped with Hammamatsu Orca/ER digital camera and BFP filter block U-MFBFPHQ, λex = 390 nm, λem = 460 nm.
  • Abe H, Wakabayashi R, Yonemura H, Yamada S, Goto M, Kamiya N.
    Split spy0128 as a potent scaffold for protein cross-linking and immobilization.
    Bioconjug Chem. 2013 Feb 20;24(2):242-50 doi: 10.1021/bc300606b
    pmid: 23350748
  • Bonekamp NA, Islinger M, Lázaro MG, Schrader M.
    Cytochemical detection of peroxisomes and mitochondria.
    Methods Mol Biol. 2013;931:467-82 doi: 10.1007/978-1-62703-056-4_24
    pmid: 23027018
  1. Protocols for dual peroxisomes and mitochondria staining.
  • Lin CP, Kim C, Smith SO, Neiman AM.
    A highly redundant gene network controls assembly of the outer spore wall in S. cerevisiae.
    PLoS Genet. 2013;9(8):e1003700 doi: 10.1371/journal.pgen.1003700
    pmid: 23966878
  • Wickersham IR, Sullivan HA, Seung HS.
    Axonal and subcellular labelling using modified rabies viral vectors.
    Nat Commun. 2013;4:2332 doi: 10.1038/ncomms3332
    pmid: 23945836
  • Chai Y, Li W, Feng G, Yang Y, Wang X, Ou G.
    Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development.
    Nat Protoc. 2012 Nov 8;7(12):2090-102 doi: 10.1038/nprot.2012.128
    pmid: 23138350
  • Salomonnson E, Mihalko LA, Verkhusha VV, Luker KE, Luker GD.
    Cell-based and in vivo spectral analysis of fluorescent proteins for multiphoton microscopy.
    J Biomed Opt. 2012 Sep 1;17(9):96001 doi: 10.1117/1.JBO.17.9.096001
    pmid: 22975677
  • Jacoby K, Metzger M, Shen BW, Certo MT, Jarjour J, Stoddard BL, Scharenberg AM.
    Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.
    Nucleic Acids Res. 2012 Jun;40(11):4954-64 doi: 10.1093/nar/gkr1303
    pmid: 22334611
  1. Constructs used: lentiviral vectors for expression of homing endonucleases linked in translation with TagBFP via a T2A self-cleaving sequence.
  2. Expression system: HEK 293T cells.
  3. Detection system: triple color flow cytometry of TagBFP, mCherry and GFP reporters on BD LSR II.
  • Dower K, Filone CM, Hodges EN, Bjornson ZB, Rubins KH, Brown LE, Schaus S, Hensley LE, Connor JH.
    Identification of a pyridopyrimidinone inhibitor of orthopoxviruses from a diversity-oriented synthesis library.
    J Virol. 2012 Mar;86(5):2632-40 doi: 10.1128/JVI.05416-11
    pmid: 22205744
  1. Constructs used: Triple reporter vaccinia virus carrying Venus, mCherry and TagBFP fluorescent proteins in a single virus under control of early (C11R), intermediate (G8R) and late (F17R) promoter respectively.
  2. Expression system: A549 cell line transduced with vaccinia virus.
  3. Detection system: fluorescence microscopy.
  • Werner ME, Hwang P, Huisman F, Taborek P, Yu CC, Mitchell BJ.
    Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells.
    J Cell Biol. 2011 Oct 3;195(1):19-26 doi: 10.1083/jcb.201106110
    pmid: 21949415
  1. Constructs used: pCS2+ vector with centrin-TagBFP fusion coding sequence (TagBFP coding sequence derived from pTagBFP-N vector).
  2. Expression system: Xenopus embryos injected with purified mRNA produced by in vitro transcription with SP6 RNA polymerase.
  3. Detection system: Simultaneous visualization of TagBFP, GFP and RFP with laser-scanning confocal microscope (A1R, Nikon).
  • Dower K, Rubins KH, Hensley LE, Connor JH.
    Development of Vaccinia reporter viruses for rapid, high content analysis of viral function at all stages of gene expression.
    Antiviral Res. 2011 Jul;91(1):72-80 doi: 10.1016/j.antiviral.2011.04.014
    pmid: 21569797
  1. Constructs used: coding sequence of TagBFP (pTagBFP-N vector) was inserted into vaccinia virus.
  2. Expression system: mammalian cell lines transduced with vaccinia virus.
  3. Detection system: Tecan Infinite M1000 fluorescent plate reader (TagBFP excitation at 415nm, emission colleted at 457 nm).
  • Walther KA, Papke B, Sinn MB, Michel K, Kinkhabwala A.
    Precise measurement of protein interacting fractions with fluorescence lifetime imaging microscopy.
    Mol Biosyst. 2011 Feb;7(2):322-36 doi: 10.1039/c0mb00132e
    pmid: 21221430
  1. Constructs used: pTagBFP-N vector.
  2. Expression system: Madin-Darby canine kidney (MDCK) cell line transiently transfected with plasmid using Effectene transfection reagent; purified recombinant TagBFP.
  3. Detection system: fluorecence lifetime imaging in vitro and in vivo with Olympus Fluoview FV 1000 confocal microscope (excitation at 405 nm, emission detected with 430 LP filter set).
  • Davis L, Maizels N.
    DNA nicks promote efficient and safe targeted gene correction.
    PLoS One. 2011;6(9):e23981 doi: 10.1371/journal.pone.0023981
    pmid: 21912657
  1. Constructs used: mammalian expression vector containing EF1α promoter, T2A translational linker and TagBFP coding sequence.
  2. Expression system: HEK 293T cells.
  3. Detection system: FACS LSR II flow cytometer, TagBFP excitation with 405 nm laser.
  • Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV.
    An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore.
    PLoS One. 2011;6(12):e28674
    pmid: 22174863
  • Bedoya L, Martínez F, Rubio L, Daròs JA.
    Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector.
    J Biotechnol. 2010 Oct 15;150(2):268-75 doi: 10.1016/j.jbiotec.2010.08.006
    pmid: 20728479
  1. Constructs used: plant expression vector based on the potyvirus Tobacco etch virus (TEV) containing TagBFP coding sequence (from pTagBFP-N vector).
  2. Expression system: wild-type tobacco plants (Nicotiana tabacum and Nicotiana benthamiana) transduced by agroinoculation with Agrobacteriam tumefaciens.
  3. Detection system: fluorescence stereomiscroscope Leica MZ 16F and confocal microscope Leica TCS-SP2-AOBS (excitation of TagBFP with 405 nm laser, emission detected at 420-470nm).
  • Subach OM, Malashkevich VN, Zencheck WD, Morozova KS, Piatkevich KD, Almo SC, Verkhusha VV.
    Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins.
    Chem Biol. 2010 Apr 23;17(4):333-41 doi: 10.1016/j.chembiol.2010.03.005
    pmid: 20416505
  1. Constructs used: pBAB/HisB-based bacterial expression vectors encoding TagBFP or TagRFP.
  2. Expression system: E. coli LMG194 starin.
  3. Detection system: crystal structure of TagBFP and TagRFP fluorescent proteins.
  • Niino Y, Hotta K, Oka K.
    Blue fluorescent cGMP sensor for multiparameter fluorescence imaging.
    PLoS One. 2010 Feb 11;5(2):e9164 doi: 10.1371/journal.pone.0009164
    pmid: 20161796
  1. Constructs used: constructed cGMP biosensor using the cGMP binding domain from a phosphodiesterase, an improved dark YFP (as a quenching acceptor) and TagBFP (as a blue fluorescent donor); TagBFP coding sequence was derived from pTagBFP-N vector.
  2. Expression system: mammalian cell lines transiently transfected with plasmid carrying the cGMP biosensor.
  3. Detection system: Olympus FluoView FV 1000 confocal laser scanning miscroscope; cGMP biosensor was observed in living cells by excitation with 405 nm laser and detection of emission at 440-480 nm.
  • Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV.
    Conversion of Red Fluorescent Protein into a Bright Blue Probe.
    Chem Biol. 2008 Oct 20;15(10):1116-24 doi: 10.1016/j.chembiol.2008.08.006
    pmid: 18940671

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