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Basic fluorescent proteins
- Multicolor protein collection for in vivo labeling applications
- Direct expression in cells
- For research and commercial use
Evrogen offers a wide collection of bright basic fluorescent proteins (FPs) for applications in the field of live-cell assays including labeling of cells, subcellular structures, and proteins; analysis of promoter activity; generation of stably transfected cell lines expressing fluorescent proteins or their fusions.
Evrogen basic fluorescent proteins are divided into subgroups according to their properties and recommended applications: |
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TagFPs
Bright monomeric fluorescent proteins of different colors for protein localization and interaction studies
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TurboFPs
Superbright and fast-maturing fluorescent proteins for gene expression analysis, cell and organelle labeling applications
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Whole body imaging
Far-red and near-infrared fluorescent proteins for whole body imaging
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BrUSLEE and SLIM
GFPs for fluorescence lifetime imaging microscopy (FLIM)
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Diversity of Evrogen basic fluorescent proteins
Ranging in color from blue to far-red, Evrogen fluorescent proteins can be used for multicolor labeling to observe different cellular events in a particular cell or a cell population.
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Normalized excitation / emission spectra
Enlarge
View in the Spectra viewer tool
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List of fluorescent proteins
| PRODUCT PAGE |
COLOR |
EX/EM MAX (nm) |
BRIGHTNESS,
% OF EGFP |
STRUCTURE |
MATURATION RATE AT 37°C |
DESCRIPTION (PDF) |
* Purified recombinant protein behaves as a pure monomer at concentrations of 10 mg/ml as verified by high-performance liquid chromatography (HPLC).
** Although NirFP is characterized by fast maturation in E. coli, due to low brightness it requires up to 48 hours for detection after transfection in mammalian cells.
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| TagFPs, protein localization tags / details  |
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| TagBFP | blue | 402 / 457 | 99 | monomer | fast |  |
| TagCFP | cyan | 458 / 480 | 64 | monomer | fast | |
| TagGFP2 | green | 483 / 506 | 105 | monomer | fast | |
| TagYFP | yellow | 508 / 524 | 94 | monomer | fast | |
| TagRFP | red (orange) | 555 / 584 | 148 | monomer | fast | |
| FusionRed | red | 580 / 608 | 53 | supermonomer* | fast | |
| mKate2 | far-red | 588 / 633 | 74 | monomer | fast | |
| TurboFPs, superbright and fast-maturing proteins / details |
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| TurboGFP | green | 482 / 502 | 112 | dimer | super fast | |
| TurboYFP | yellow | 525 / 538 | 169 | dimer | super fast | |
| TurboRFP | red (orange) | 553 / 574 | 187 | dimer | super fast | |
| TurboFP602 | true-red | 574 / 602 | 79 | dimer | fast | |
| TurboFP635 | far-red | 588 / 635 | 67 | dimer | super fast | |
| Fluorescent proteins for whole body imaging / details |
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| Katushka2S | far-red | 588 / 633 | 89 | dimer | super fast | |
| TurboFP650 | near-infrared | 592 / 650 | 47 | dimer | super fast | |
| NirFP | near-infrared | 605 / 670 | 13 | dimer | fast** | |
| Fluorescent proteins for fluorescence lifetime imaging microscopy (FLIM) / details |
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| BrUSLEE | green | 487 / 509 | 78 | | fast | |
| SLIM | green | 488 / 508 | 13 | | fast | |
| Old or discontinued variants |
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| TagGFP | green | 482 / 505 | 104 | monomer | fast | |
| TagFP635 | far-red | 588 / 635 | 45 | monomer | fast | |
| JRed | true-red | 584 / 610 | 26 | weak dimer | slow | |
| PhiYFP | yellow | 525 / 537 | 158 | dimer | fast | |
| CopGFP | green | 482 / 502 | 126 | tetramer | super fast | |
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