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Fluorescent proteins for FLIM

- Green fluorescence
- Fast maturation and high photostability
- Recommended for fluorescence lifetime imaging microscopy (FLIM)

BrUSLEE and SLIM can be used in Fluorescence Lifetime Imaging (FLIM) where the contrast is based on the lifetime of individual fluorescent proteins rather than their emission spectra. This proteins can be easily expressed and detected in a wide range of organisms. No cytotoxic effects or visible protein aggregation are observed.

Evrogen offers expression vectors encoding green fluorescent proteins for FLIM.

Spectral diversity

BrUSLEE

SLIM

Normalized excitation / emission spectra

BrUSLEE spectra SLIM spectra
Available proteins
CHARACTERISTICBrUSLEESLIM
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa2727
Polypeptide length, aa239239
Fluorescence colorgreengreen
Excitation maximum, nm487488
Emission maximum, nm509508
Quantum yield0.30.06
Extinction coefficient, M-1cm-186 00070 000
Brightness*264.2
Brightness, % of EGFP7813
Fluorescence lifetime, ps8001300
Fluorescence intensity decaynearly single-exponentialnearly single-exponential
Aggregationnono
Maturation rate at 37°Cfastfast
Photostabilityhighhigh
Cell toxicitynot observednot observed
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