Whole body imaging

- Bright fluorescence
- Excellent performance in whole body imaging
- For research and commercial use

Deep-tissue imaging using fluorescent proteins allows direct and non-invasive observation of the biological processes inside living organisms. The major difficulty of fluorescent imaging within whole animals is the light absorption by melanin and hemoglobin, as well as light scattering. Both absorption and scattering become less pronounced as the light wavelength increases. The optimal "optical window", which is most transparent for the visualization in living tissues, is considered to be between 650-700 and 1100 nm. Therefore, use of far-red or near infrared fluorescent proteins drastically increases sensitivity of the whole body imaging comparing to conventional FPs.

Evrogen offers expression vectors encoding far-red and near infrared fluorescent proteins perfectly suitable for such application.

Spectral diversity

Whole body imaging excitation spectra. Whole body imaging emission spectra.

Normalized excitation / emission spectra

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Available proteins
CHARACTERISTIC Katushka2STurboFP650NirFP
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
** Although NirFP is characterized by fast maturation in E. coli, due to low brightness it requires up to 48 hours for detection after transfection in mammalian cells.
Fluorescence color far-red near-infrared near-infrared
Excitation max (nm) 588 592 605
Emission max (nm) 633 650 670
Quantum yield 0.44 0.24 0.06
Ext. coeff. (M-1cm-1) 67 000 65 000 70 000
Brightness* 29.5 15.6 4.2
Brightness, % of EGFP 89 47 13
pKa 5.4 5.7 4.5
Structure dimer dimer dimer
Cell toxicity not observed not observed not observed
Aggregation no no no
Maturation rate at 37°C super fast super fast fast**
Molecular weight (kDa) 26 26 26
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