Katushka2S

Far-red fluorescent protein Katushka2S

- Super bright far-red fluorescence
- High signal-to-noise ratio, fast maturation
- Recommended for whole body imaging

Katushka2S is the next generation of far-red fluorescent protein TurboFP635 (Katushka) [Luker et al., 2015; Shcherbo et al., 2009; Gurskaya et al., 2011]. Katushka2S exhibits highest brightness, fastest maturation and superior signal-to-noise ratio compared to TurboFP635 and many other available far-red fluorescent proteins: E2-Crimson, mNeptune, mNeptune2.5, mCardinal, TurboFP650 and NirFP [Luker et al., 2015], which makes it the protein of choice for visualization within living tissues. Katushka2S can also be used together with iRFP720 [Luker et al., 2015] for dual color whole body imaging.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Katushka2S expression/source vectors

pKatushka2S-C FP761 Mammalian expression vector encoding humanized Katushka2S and allowing its expression and generation of fusions to the Katushka2S C-terminus 20 μg € 480 / 240*
pKatushka2S-N FP762 Mammalian expression vector encoding humanized Katushka2S and allowing its expression and generation of fusions to the Katushka2S N-terminus 20 μg € 480 / 240*
pKatushka2S-B FP763 Bacterial expression vector; source of the Katushka2S coding sequence 20 μg € 480 / 240*


Antibody against Katushka2S

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Gurskaya NG, Staroverov DB, Fradkov AF, Lukyanov KA. Coding region of far-red fluorescent protein katushka contains a strong donor splice site. Bioorg Khim. 2011; 37 (3):425-8. (in Russian) / pmid: 21899059
  • Luker KE, Pata P, Shemiakina II, Pereverzeva A, Stacer AC, Shcherbo DS, Pletnev VZ, Skolnaja M, Lukyanov KA, Luker GD, Pata I, Chudakov DM. Comparative study reveals better far-red fluorescent protein for whole body imaging. Sci Rep. 2015; 5 :10332. doi: 10.1038/srep10332 / pmid: 26035795
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
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