TurboYFP

Yellow fluorescent protein TurboYFP

- Super bright true-yellow fluorescence
- Emission wavelength is ideally positioned between those of green and red fluorescent proteins
- Fast maturation, high pH-stability and photostability
- Destabilized variant is available
- Recommended for gene expression analysis and cell labeling

TurboYFP is an enhanced variant of the yellow fluorescent protein PhiYFP from jellyfish Phialidium sp. [Shagin et al., 2004]. It possesses superbright yellow fluorescence with emission maximum at 538 nm and is ideally positioned between green and red fluorescent proteins, allowing easy separation from these markers by flow cytometry using common channels of detection and a single laser excitation line. Compared with Phi-Yellow proteins, TurboYFP matures faster in mammalian cells.

TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell labeling and tracking the promoter activity. Destabilized TurboYFP variant allows analysis of rapid and/or transient events in gene regulation.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


TurboYFP expression/source vectors

pTurboYFP-C FP611 Mammalian expression vector encoding humanized TurboYFP and allowing its expression and generation of fusions to the TurboYFP C-terminus 20 μg € 320 / 160*
pTurboYFP-N FP612 Mammalian expression vector encoding humanized TurboYFP and allowing its expression and generation of fusions to the TurboYFP N-terminus 20 μg € 320 / 160*
pTurboYFP-B FP613 Bacterial expression vector; source of the TurboYFP coding sequence 20 μg € 320 / 160*
pTurboYFP-PRL FP615 Promoterless vector encoding humanized TurboYFP and designed for monitoring of activity of different promoters and promoter/enhancer combinations 20 μg € 320 / 160*
peTurboYFP-PRL-dest1 FP616 Promoterless vector encoding destabilized TurboYFP and designed for monitoring of activity of different promoters and promoter/enhancer combinations 20 μg € 320 / 160*
peTurboYFP-dest1 FP617 Mammalian expression vector encoding destabilized TurboYFP for its expression and generation of fusions to the TurboYFP-dest1 N-terminus 20 μg € 320 / 160*


Antibody against TurboYFP

Anti-TurboYFP
antibody
AB605 Rabbit polyclonal antibody against TurboYFP, PhiYFP and PhiYFP-m 100 μg € 150
AB606 200 μg € 200

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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