NirFP

Near-infrared fluorescent protein NirFP

- Near-infrared fluorescence with emission maximum at 670 nm
- Extremely high photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for multicolor applications

NirFP (scientific name eqFP670) is a red-shifted variant of TurboFP635 (Katushka) [Shcherbo et al., 2010]. NirFP is characterized by a strong bathochromic shift, with excitation and emission peaks at 605 nm and 670 nm, respectively. It is currently the most red-shifted fluorescent protein available, with approximately half of emission falling in the infrared part of the spectrum. The brightness of NirFP in the 700 – 900 nm region upon excitation at 635 nm is about 4 times higher than the brightness of TurboFP635 and 1.6 times higher than the brightness of TurboFP650. The protein does not show residual short wavelength fluorescence of intermediate or alternative chromophore forms, in contrast to E2-Crimson [Strack et al., 2009], which exhibits a second bright blue emission peak, and mNeptune [Lin et al., 2009], which has a pronounced green peak.

NirFP is characterized by high pH stability and extremely high photostability that should allow for accumulation of the fluorescent signal over long exposure times.

NirFP is recommended for multicolor applications. It can also be used for whole body imaging utilizing long wavelengths for excitation (e.g., 633 or 635 nm laser lines).

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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NirFP expression/source vectors

pNirFP-C FP741 Mammalian expression vector encoding humanized NirFP and allowing its expression and generation of fusions to the NirFP C-terminus 20 μg € 400 / 200*
peNirFP-N FP743 Mammalian expression vector encoding humanized NirFP and allowing its expression and generation of fusions to the NirFP N-terminus 20 μg € 400 / 200*


Antibody against NirFP

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Lin MZ, McKeown MR, Ng HL, Aguilera TA, Shaner NC, Campbell RE, Adams SR, Gross LA, Ma W, Alber T, Tsien RY. Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Chem Biol. 2009; 16 (11):1169-79. doi: 10.1016/j.chembiol.2009.10.009 / pmid: 19942140
  • Shcherbo D, Shemiakina II, Ryabova AV, Luker KE, Schmidt BT, Souslova EA, Gorodnicheva TV, Strukova L, Shidlovskiy KM, Britanova OV, Zaraisky AG, Lukyanov KA, Loschenov VB, Luker GD, Chudakov DM. Near-infrared fluorescent proteins. Nat Methods. 2010; 7 (10):827-9. doi: 10.1038/nmeth.1501 / pmid: 20818379
  • Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS. A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Biochemistry. 2009; 48 (35):8279-81. doi: 10.1021/bi900870u / pmid: 19658435
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