PhiYFP

Phi-Yellow fluorescent proteins

- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines

PhiYFP and PhiYFP-m are the mutants of a natural yellow fluorescent protein from Phialidium sp. (Cnidaria; Hydrozoa; Hydroida; Leptomedusae; Campanulariidae) [Shagin et al., 2004].

Phi-Yellow proteins have excitation/emission maxima at 525 and 537 nm, respectively. They exhibit lower brightness and maturation rate than TurboYFP, but are more suitable for generation of stably transfected cell lines. The emission wavelength of these proteins is ideally positioned between those of green and red fluorescent proteins, allowing easy separation of these fluorescent tags by flow cytometry using common channels of detection and a single laser excitation line.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


PhiYFP expression/source vectors

pPhi-Yellow-C FP601 Mammalian expression vector encoding humanized PhiYFP-m and allowing its expression and generation of fusions to the PhiYFP-m C-terminus 20 μg € 320 / 160*
pPhi-Yellow-N FP602 Mammalian expression vector encoding humanized PhiYFP and allowing its expression and generation of fusions to the PhiYFP N-terminus 20 μg € 320 / 160*
pPhi-Yellow-B FP603 Bacterial expression vector; source of the PhiYFP coding sequence 20 μg € 320 / 160*
pPhi-Yellow-PRL FP604 Promoterless vector encoding humanized PhiYFP and designed for monitoring of activity of different promoters and promoter/enhancer combinations 20 μg € 320 / 160*
pPhi-Yellow-peroxi FP606 Mammalian expression vector encoding humanized PhiYFP-m targeted to peroxisomes 20 μg € 320 / 160*
pPhi-Yellow-mito FP607 Mammalian expression vector encoding humanized PhiYFP targeted to mitochondria 20 μg € 320 / 160*


Recombinant protein

rPhiYFP FP651 Purified recombinant bright yellow fluorescent protein 100 μg € 130


Antibody against PhiYFP

Anti-TurboYFP
antibody
AB605 Rabbit polyclonal antibody against TurboYFP, PhiYFP and PhiYFP-m 100 μg € 150
AB606 200 μg € 200

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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