FusionRed

Red fluorescent protein FusionRed

- Superior performance in fusions
- Low cytotoxicity
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and long-term experiments

FusionRed is a red fluorescent protein characterized by improved performance in fusions and low toxicity [Shemiakina et al., 2012]. FusionRed lacks the residual tendency of other monomeric RFPs to dimerize at high concentration and behaves as a pure monomer at concentrations up to 10 mg/ml in HPLC analysis. Such "supermonomeric" properties ensure superior efficiency of FusionRed in protein labeling applications, especially in the cells with high expression level.

Similarly to parental mKate2, FusionRed demonstrates fast maturation rate, high pH-stability and photostability, and significantly lower cytotoxicity than widely used mCherry [Shaner et al., 2004] and mRuby [Kredel et al., 2009].

FusionRed is mainly intended for protein labeling and long-term experiments including generation of transgenic animals.

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FusionRed expression/source vectors

pFusionRed-C FP411 Mammalian expression vector encoding humanized FusionRed and allowing its expression and generation of fusions to the FusionRed C-terminus 20 μg € 480 / 240*
pFusionRed-N FP412 Mammalian expression vector encoding humanized FusionRed and allowing its expression and generation of fusions to the FusionRed N-terminus 20 μg € 480 / 240*
pFusionRed-actin FP413 Mammalian expression vector encoding humanized FusionRed fused with human cytoplasmic β-actin 20 μg € 480 / 240*
pFusionRed-annexin FP414 Mammalian expression vector encoding humanized FusionRed fused with human annexin 20 μg € 480 / 240*
pFusionRed-CD151 FP415 Mammalian expression vector encoding humanized FusionRed fused with human CD151 20 μg € 480 / 240*
pFusionRed-Cx26 FP416 Mammalian expression vector encoding humanized FusionRed fused with rat connexin 26 20 μg € 480 / 240*
pFusionRed-Cx43 FP417 Mammalian expression vector encoding humanized FusionRed fused with rat connexin 43 20 μg € 480 / 240*
pFusionRed-f-mem FP418 Mammalian expression vector encoding membrane-targeted FusionRed 20 μg € 480 / 240*
pFusionRed-Golgi FP419 Mammalian expression vector encoding humanized FusionRed fused with human Golgi targeting sequence (GTS) 20 μg € 480 / 240*
pFusionRed-ER FP420 Mammalian expression vector encoding humanized FusionRed targeted to the endoplasmic reticulum 20 μg € 480 / 240*
pFusionRed-H2B FP421 Mammalian expression vector encoding humanized FusionRed fused with human histone H2B 20 μg € 480 / 240*
pFusionRed-laminB1 FP422 Mammalian expression vector encoding humanized FusionRed fused with human lamin B1 20 μg € 480 / 240*
pFusionRed-vimentin FP423 Mammalian expression vector encoding humanized FusionRed fused with human cytokeratin-18 20 μg € 480 / 240*
pFusionRed-vinculin FP424 Mammalian expression vector encoding humanized FusionRed fused with human vinculin 20 μg € 480 / 240*
pFusionRed-zyxin FP425 Mammalian expression vector encoding humanized FusionRed fused with human zyxin 20 μg € 480 / 240*
pFusionRed-actinin FP426 Mammalian expression vector encoding humanized FusionRed fused with human α-actinin 20 μg € 480 / 240*
pFusionRed-endo FP427 Mammalian expression vector encoding humanized FusionRed fused with human RhoB protein 20 μg € 480 / 240*
pFusionRed-MAP4 FP428 Mammalian expression vector encoding humanized FusionRed fused with microtubule binding domain of mouse microtubule-associated protein 4 (MAP4) 20 μg € 480 / 240*
pFusionRed-paxillin FP429 Mammalian expression vector encoding humanized FusionRed fused with chicken paxillin 20 μg € 480 / 240*
pFusionRed-PDHA1 FP430 Mammalian expression vector encoding humanized FusionRed fused with human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1) 20 μg € 480 / 240*
pFusionRed-Rab5a FP431 Mammalian expression vector encoding humanized FusionRed fused with human Ras-related protein Rab-5A 20 μg € 480 / 240*
pFusionRed-talin FP432 Mammalian expression vector encoding humanized FusionRed fused with mouse talin-1 protein 20 μg € 480 / 240*
pFusionRed-tubulin FP433 Mammalian expression vector encoding humanized FusionRed fused with human α-tubulin 20 μg € 480 / 240*
pFusionRed-cadherin FP434 Mammalian expression vector encoding humanized FusionRed fused with human VE-cadherin 20 μg € 480 / 240*
pFusionRed-CDC42 FP435 Mammalian expression vector encoding humanized FusionRed fused with human CDC42 20 μg € 480 / 240*
pFusionRed-myosin FP436 Mammalian expression vector encoding humanized FusionRed fused with mouse myosin regulatory light polypeptide 9 20 μg € 480 / 240*
pFusionRed-PCNA FP437 Mammalian expression vector encoding humanized FusionRed fused with human PCNA 20 μg € 480 / 240*
pFusionRed-B FP438 Bacterial expression vector; source of the FusionRed coding sequence 20 μg € 480 / 240*


Antibody against FusionRed

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Kredel S, Oswald F, Nienhaus K, Deuschle K, Röcker C, Wolff M, Heilker R, Nienhaus GU, Wiedenmann J. mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PLoS One. 2009; 4 (2):e4391. doi: 10.1371/journal.pone.0004391 / pmid: 19194514
  • Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
  • Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D. A monomeric red fluorescent protein with low cytotoxicity. Nat Commun. 2012; 3 :1204. doi: 10.1038/ncomms2208 / pmid: 23149748
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