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Blue fluorescent protein TagBFP
- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications
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TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
Compared to EBFP2 [Ai et al., 2007], TagBFP is more then 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of TagBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.
Good overlap between the emission spectrum of TagBFP and the absorbance spectra of TagGFP2 allows using these two proteins as a FRET pair.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | | The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| TagBFP expression/source vectors |
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| pTagBFP-C |
FP171 |
Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP C-terminus |
20 μg |
€ 400 |
| pTagBFP-N |
FP172 |
Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP N-terminus |
20 μg |
€ 400 |
| pTagBFP-actin |
FP174 |
Mammalian expression vector encoding humanized TagBFP fused with human cytoplasmic β-actin |
20 μg |
€ 400 |
| pTagBFP-tubulin |
FP175 |
Mammalian expression vector encoding humanized TagBFP fused with human α-tubulin |
20 μg |
€ 400 |
| pTagBFP-H2B |
FP176 |
Mammalian expression vector encoding humanized TagBFP fused with human histone H2B |
20 μg |
€ 400 |
| Gateway® TagBFP-AS-C entry clone |
FP177 |
Gateway® entry clone for generation of fusions to the C-terminus of TagBFP; transfer of the constract encoding TagBFP or its fusion into Gateway® destination vectors; TagBFP codon usage is optimized for expression in Arabidopsis and Saccharomyces |
20 μg |
€ 400 |
| Gateway® TagBFP-AS-N entry clone |
FP178 |
Gateway® entry clone for generation of fusions to the N-terminus of TagBFP; transfer of the constract encoding TagBFP or its fusion into Gateway® destination vectors; TagBFP codon usage is optimized for expression in Arabidopsis and Saccharomyces |
20 μg |
€ 400 |
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| Vector set |
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| Fusion Blue |
FPF20 |
Mammalian expression vectors encoding TagBFP for its expression and fusion generation: pTagBFP-N and pTagBFP-C |
20 μg each |
€ 650 |
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| Antibody against TagBFP |
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Anti-tRFP
antibody |
AB233 |
Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagBFP, TagRFP, TagFP635, and mKate2 |
100 μg |
€ 150 |
| AB234 |
200 μg |
€ 200 |
References:
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Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE.
Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins.
Biochemistry. 2007; 46 (20):5904-10. / pmid: 17444659
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Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV.
Conversion of Red Fluorescent Protein into a Bright Blue Probe.
Chem Biol. 2008; 15 (10):1116-24. / pmid: 18940671
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