TagBFP

Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications

TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.

Compared to EBFP2 [Ai et al., 2007], TagBFP is more then 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of TagBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.

Good overlap between the emission spectrum of TagBFP and the absorbance spectra of TagGFP2 allows using these two proteins as a FRET pair.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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TagBFP expression/source vectors

pTagBFP-C FP171 Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP C-terminus 20 μg € 400 / 200*
pTagBFP-N FP172 Mammalian expression vector encoding humanized TagBFP and allowing its expression and generation of fusions to the TagBFP N-terminus 20 μg € 400 / 200*
pTagBFP-actin FP174 Mammalian expression vector encoding humanized TagBFP fused with human cytoplasmic β-actin 20 μg € 400 / 200*
pTagBFP-tubulin FP175 Mammalian expression vector encoding humanized TagBFP fused with human α-tubulin 20 μg € 400 / 200*
pTagBFP-H2B FP176 Mammalian expression vector encoding humanized TagBFP fused with human histone H2B 20 μg € 400 / 200*
Gateway® TagBFP-AS-C entry clone FP177 Gateway® entry clone for generation of fusions to the C-terminus of TagBFP; transfer of the constract encoding TagBFP or its fusion into Gateway® destination vectors; TagBFP codon usage is optimized for expression in Arabidopsis and Saccharomyces 20 μg € 400 / 200*
Gateway® TagBFP-AS-N entry clone FP178 Gateway® entry clone for generation of fusions to the N-terminus of TagBFP; transfer of the constract encoding TagBFP or its fusion into Gateway® destination vectors; TagBFP codon usage is optimized for expression in Arabidopsis and Saccharomyces 20 μg € 400 / 200*


Antibody against TagBFP

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE. Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry. 2007; 46 (20):5904-10. / pmid: 17444659
  • Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV. Conversion of Red Fluorescent Protein into a Bright Blue Probe. Chem Biol. 2008; 15 (10):1116-24. doi: 10.1016/j.chembiol.2008.08.006 / pmid: 18940671
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