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Hydrogen peroxide sensor HyPer
- Ratiometric detection of intracellular H2O2 level changes
- High selectivity and sensitivity, no artifactual ROS generation
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring H2O2 production inside living cells
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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HyPer-AS variant
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HyPer-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clone.
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Gateway® HyPer-AS entry clone*
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FP943
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HyPer-Cyto
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HyPer comprises fluorescent part which codon usage is optimized for high expression in mammalian cells [Haas et al., 1996] and OxyR part from E. coli with autogenic codon usage. Effective expression of HyPer occures in both bacteria and mammalian cells.
When expressed in mammalian cells, this variant is evenly distributed throughout the cytosol and the nucleus.
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pHyPer-cyto*
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FP941
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HyPer-dMito
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Two copies of mitochondrial targeting sequence derived from subunit VIII precursor of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995] are fused to HyPer N-terminus. When expressed in mammalian cells, this variant is localized in mitochondria.
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pHyPer-dMito*
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FP942
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HyPer-nuc
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Three copies of the nuclear localization signal [Fischer-Fantuzzi and Vesco, 1988] are fused to the HyPer C-terminus. When expressed in mammalian cells, this variant is localized in the nuclei.
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pHyPer-nuc*
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FP944
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References:
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Fischer-Fantuzzi L, Vesco C.
Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.
Mol Cell Biol. 1988; 8 (12):5495-503. / pmid: 2854199
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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