Hydrogen peroxide sensor HyPer

- Ratiometric detection of intracellular H₂O₂ level changes
- High selectivity and sensitivity, no artifactual ROS generation
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring H₂O₂ production inside living cells

Main properties

HyPer excitation (thin line) and emission (thick line) spectra. Download HyPer spectra (xls)	CHARACTERISTIC   Emission maximum, nm 516 Excitation maximum, nm 420 and 500 Fluorescence color green Polypeptide length, aa 478 Molecular weight, kDa 52 Specificity H2O2 Sensitivity submicromolar H2O2 concentrations pKa 8.5 Structure monomer Aggregation no Maturation rate at 37°C fast