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    HyPer

Hydrogen peroxide sensor HyPer

- Ratiometric detection of intracellular H2O2 level changes
- High selectivity and sensitivity, no artifactual ROS generation
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring H2O2 production inside living cells

Main properties

HyPer spectra

HyPer excitation (thin line) and emission (thick line) spectra.

Download HyPer spectra (xls)

CHARACTERISTIC

 

Emission maximum, nm516
Excitation maximum, nm420 and 500
Fluorescence colorgreen
Polypeptide length, aa478
Molecular weight, kDa52
SpecificityH2O2
Sensitivitysubmicromolar H2O2 concentrations
pKa8.5
Structuremonomer
Aggregationno
Maturation rate at 37°Cfast
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