TagBFP

Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Performance and use

TagBFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagBFP expression vectors produce bright fluorescence in 10-12 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.

TagBFP performance in fusions has been demonstrated in the β-actin and α-tubulin models. It can be used in multicolor labeling applications with cyan, green, yellow, red, and far-red fluorescent dyes.

High pH-stability with pKa=2.7 makes it possible to use TagBFP for imaging in acidic organelles, such as late and recycling endosomes and lysosomes.

HeLa cells expressing TagBFP fusion proteins.

Emission spectrum of TagBFP and absorbance spectra of GFPs demonstrate good overlap, suggesting TagBFP may be a good donor for GFP acceptor for Forster resonance energy transfer (FRET). Among available GFPs, TagGFP2 is prefferable because of the small absorbance below 400 nm where TagBFP is excited. High efficiency of TagBFP-TagGFP2 FRET pair was demonstrated in living cells [Subach et al., 2008].

References:

  • Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV. Conversion of Red Fluorescent Protein into a Bright Blue Probe. Chem Biol. 2008; 15 (10):1116-24. doi: 10.1016/j.chembiol.2008.08.006 / pmid: 18940671
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