TagBFP normalized excitation (thin line) and emission (thick line) spectra.
Spectra viewer tool Download TagBFP spectra (xls)
| CHARACTERISTIC | |
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| Molecular weight, kDa | 26 | Polypeptide length, aa | 233 | Fluorescence color | blue | Excitation maximum, nm | 402 | Emission maximum, nm | 457 | Quantum yield | 0.63 | Extinction coefficient, M-1cm-1 | 52 000 | Brightness* | 32.8 | Brightness, % of EGFP | 99 | pKa | 2.7 | Structure | monomer | Aggregation | no | Maturation rate at 37°C | fast | Photostability | high | Cell toxicity | not observed | Main advantages | bright blue monomeric fluorescent protein | Possible limitations | not observed |
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