TagBFP

Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagBFP spectra

TagBFP normalized excitation (thin line) and emission (thick line) spectra.

Spectra viewer tool
Download TagBFP spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26
Polypeptide length, aa233
Fluorescence colorblue
Excitation maximum, nm402
Emission maximum, nm457
Quantum yield0.63
Extinction coefficient, M-1cm-152 000
Brightness*32.8
Brightness, % of EGFP99
pKa2.7
Structuremonomer
Aggregationno
Maturation rate at 37°Cfast
Photostabilityhigh
Cell toxicitynot observed
Main advantagesbright blue monomeric fluorescent protein
Possible limitationsnot observed
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