TagBFP

Blue fluorescent protein TagBFP

- Bright blue fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high photostability
- Extremely high pH-stability
- Recommended for protein labeling, acidic organelle labeling, FRET applications


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized TagBFP TagBFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pTagBFP-C FP171
pTagBFP-N FP172
TagBFP-AS variant TagBFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones. Gateway® TagBFP-AS-C entry clone FP177
Gateway® TagBFP-AS-N entry clone FP178
TagBFP-actin fusion Human β-actin is fused to the TagBFP C-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of β-actin in living cells. pTagBFP-actin FP174
TagBFP-tubulin fusion Human α-tubulin is fused to the TagBFP C-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of α-tubulin in living cells. pTagBFP-tubulin FP175
TagBFP-H2B fusion Human histone H2B is fused to the TagBFP N-terminus. When expressed in mammalian cells, this fusion provides blue fluorescent labeling of histone H2B in living cells. pTagBFP-H2B FP176


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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