The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.
pTagBFP-H2B is a mammalian expression vector encoding TagBFP-H2B fusion protein (see reporter description). The vector can be used for fluorescent labeling of histone H2B in living cells.
TagBFP codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996]. Human histone H2B is fused to the TagBFP N-terminus.
pTagBFP-H2B vector can be used as a source of TagBFP-H2B hybrid sequence. The vector backbone contains unique restriction sites that permit its excision and further insertion into expression vector of choice.
Note: The plasmid DNA was isolated from dam+-methylated
The vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells expressing SV40 T-antigen, pUC origin of replication for propagation in
SV40 early promoter (PSV40) provides neomycin resistance gene (Neor) expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression (Kanr) in
Expression in mammalian cells
pTagBFP-H2B vector can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of the TagBFP-H2B fusion in eukaryotic cells. If required, stable transformants can be selected using G418 [Gorman, 1985].
Suitable host strains for propagation in
Location of features
PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
H2B-TagBFP fusion: 657-1751
Histone H2B protein: 657-1034
Last amino acid in H2B: 1032-1034
Stop codon: 1752-1754
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1908-1913 & 1937-1942
mRNA 3' ends: 1946 & 1958
f1 single-strand DNA origin: 2005-2460
Bacterial promoter for expression of Kanr gene
-35 region: 2522-2527
-10 region: 2545-2550
Transcription start point: 2557
SV40 origin of replication: 2801-2936
SV40 early promoter
Enhancer (72-bp tandem repeats): 2634-2705 & 2706-2777
21-bp repeats: 2781-2801, 2802-2822 & 2824-2844
Early promoter element: 2857-2863
Major transcription start points: 2853, 2891, 2897 & 2902
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2985-2987
Stop codon: 3777-3779
G->A mutation to remove Pst I site: 3167
C->A (Arg to Ser) mutation to remove BssH II site: 3513
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4015-4020 & 4028-4033
pUC plasmid replication origin: 4364-5007
TagBFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.
The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.
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