TurboFP602

True-red fluorescent protein TurboFP602

- Bright true-red fluorescence
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for gene expression analysis and cell and organelle labeling in an autofluorescent environment

TurboFP602 is a red-shifted variant of the red fluorescent protein TurboRFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. TurboFP602 possesses true-red fluorescence (with excitation/emission maxima at 574/602 nm, respectively), optimal for detection via most popular filter sets, and is easily distinguished from background signals. TurboFP602 exhibits fast maturation and high pH stability.

TurboFP602 is mainly intended for applications where fast appearance of true-red fluorescence is crucial. It is specially recommended for cell and organelle labeling and for tracking the promoter activity in autofluorescent tissues.

Main properties

TurboFP602 spectra

TurboFP602 normalized excitation (thin line) and emission (thick line) spectra.

Spectra viewer tool
Download TurboFP602 spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa26.28
Polypeptide length, aa231
Fluorescence colortrue-red
Excitation maximum, nm574
Emission maximum, nm602
Quantum yield0.35
Extinction coefficient, M-1cm-174 400
Brightness*26.0
Brightness, % of EGFP79
pKa4.7
Structuredimer
Aggregationno
Maturation rate at 37°Cfast
Photostabilitymedium
Cell toxicitynot observed
Main advantagestrue-red fluorescent protein, ideal compatibility with popular filter sets
Possible limitationsdimer, limited applicability for fusions generation

Recommended filter sets and antibodies

TurboFP602 can be recognized using Anti-tRFP antibody (Cat.# AB233-AB234) available from Evrogen.

TurboFP602 can be detected using TRITC filter set or similar. Recommended Omega Optical filter sets are QMAX-Red and XF102-2.

Performance and use

TurboFP602 can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TurboFP602 expression vectors produce bright fluorescence in 8-12 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.

Despite its dimeric structure, TurboFP602 performs well in some fusions. However, for protein labeling applications we recommend using specially optimized monomeric TagFPs.

TurboFP602 suitability to generate stably transfected cells has been proven by Marinpharm company. Cell lines expressing TurboFP602 are commercially available.

TurboFP602 can be used in multicolor labeling applications with blue, cyan, green, and yellow fluorescent dyes.

TurboFP602 expression in mammalian cells.

(A) Transiently transfected Hela cells; (B) transiently transfected HeLa cells expressing mitochondria-targeted TurboFP602; (C) stably transfected human melanoma cell line Mel-Juso.

Image (C) was kindly provided by Dr. Christian Petzelt (Marinpharm).


Available variants and fusions
VariantDescriptionRelated vectorCat.#Click for image
Humanized TurboFP602 TurboFP602 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. pTurboFP602-C FP711
pTurboFP602-N FP712
pTurboFP602-B FP713
pTurboFP602-PRL FP715
TurboFP602-mito fusion A mitochondrial targeting sequence (MTS) is fused to the TurboFP602 N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides true-red fluorescent labeling of mitochondria. pTurboFP602-mito FP717


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
  • Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
  • Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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