The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.
|BamH I||Nco I*|
|Eag I||Hind III|
* – not unique site.
pKatushka2S-B is a prokaryotic expression vector encoding far-red fluorescent protein Katushka2S (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].
The vector is primarily intended as a source of Katushka2S coding sequence. Flanking restriction sites are convenient for excision of Katushka2S sequence and its further insertion into other expression vectors of choice. Alternatively, Katushka2S coding sequence can be amplified by PCR.
Note: The plasmid DNA was isolated from dam+-methylated
The vector can be also used for Katushka2S expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in
Location of features
T5 promoter/lac operator element: 7-87
T5 transcription start: 61
Katushka2S coding sequence: 147-854
Lambda t0 transcriptional termination region: 884-978
rrnB T1 transcriptional termination region: 1740-1838
ColE1 origin of replication: 2314
beta-lactamase coding sequence: 3932-3072
Katushka2S-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 8,138,320; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.
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