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Red fluorescent protein TagRFP
- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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Humanized TagRFP
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TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pTagRFP-C
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FP141
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pTagRFP-N
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FP142
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TagRFP-AS variant
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TagRFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones.
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Gateway® TagRFP-AS-C entry clone
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FP148
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Gateway® TagRFP-AS-N entry clone
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FP149
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TagRFP-tubulin fusion
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Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-tubulin in living cells.
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pTagRFP-tubulin
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FP145
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TagRFP-mito fusion
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A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria.
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pTagRFP-mito
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FP147
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TagRFP-Cx43 fusion
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Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 43 in living cells.
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pTagRFP-Cx43
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FP364
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TagRFP-Golgi fusion
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Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of Golgi apparatus.
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pTagRFP-Golgi
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FP367
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TagRFP-H2B fusion
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Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of histone H2B in living cells.
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pTagRFP-H2B
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FP368
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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