Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications

Main properties

TagRFP normalized excitation (thin line) and emission (thick line) spectra. Spectra viewer tool Download TagRFP spectra (xls)

CHARACTERISTIC * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. ** Time to bleach 50% of fluorescent signal brightness. Molecular weight, kDa 27 Polypeptide length, aa 237 Fluorescence color red (orange) Excitation maximum, nm 555 Emission maximum, nm 584 Quantum yield 0.48 Extinction coefficient, M-1cm-1 100 000 Brightness* 48.0 Brightness, % of EGFP 148 pKa 3.8 Structure monomer Aggregation no Maturation half-time, min 100 Photostability, widefield** 48 Photostability, confocal** 125 Cell toxicity not observed Main advantages bright red monomeric fluorescent protein Possible limitations medium photostability