Ranging in color from blue to far-red, Evrogen fluorescent proteins can
be used in fluorescence resonance energy transfer (FRET) applications as
donors and acceptors of fluorescence. TagFPs are perfect for in
vivo protein interaction studies by FRET due to their improved
performance in fusions.
TagBFP-TagGFP2 and TagGFP2-TagRFP pairs show the
highest FRET efficiency among the tested TagFPs combinations and compare
favorably to other available FRET pairs of monomeric fluorescent proteins.