FRET-based applications

- High effective FRET pairs

Ranging in color from blue to far-red, Evrogen fluorescent proteins can be used in fluorescence resonance energy transfer (FRET) applications as donors and acceptors of fluorescence. TagFPs are perfect for in vivo protein interaction studies by FRET due to their improved performance in fusions.

TagBFP-TagGFP2 and TagGFP2-TagRFP pairs show the highest FRET efficiency among the tested TagFPs combinations and compare favorably to other available FRET pairs of monomeric fluorescent proteins.

Recommended pairs for FRET applications
PAIR	DONOR	ACCEPTOR	CALCULATED FORSTER DISTANCE (Ro)	FRET EFFICIENCY (E)

TagBFP-TagGFP2	TagBFP ex/em max 402/457 nm	TagGFP2 ex/em max 483/506 nm	5.3 nm	0.57
TagGFP2-TagRFP	TagGFP2 ex/em max 483/506 nm	TagRFP ex/em max 555/584 nm	5.7 nm	0.50

Spectral overlap for TagBFP-TagGFP2 and TagGFP2-TagRFP FRET pairs.

Excitation (thin lines) and emission (thick lines) spectra of TagBFP (blue), TagGFP2 (green) and TagRFP (red) are shown individually. Spectral overlap is filled with gray.


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