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Phi-Yellow fluorescent proteins
- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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PhiYFP
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PhiYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. PhiYFP allows generation of fusions to its N-terminus but unsuited to generate C-terminal fusions.
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pPhi-Yellow-N
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FP602
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pPhi-Yellow-PRL
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FP604
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PhiYFP-mito fusion
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A mitochondrial targeting sequence (MTS) is fused to the PhiYFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria.
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pPhi-Yellow-mito
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FP607
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PhiYFP-m-peroxi fusion
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Peroxisomal targeting signal [Gould et al., 1989] encoding tripeptide SKL was fused to the 3' end of PhiYFP-m sequence. This tripeptide targets the fusion protein to the matrix of peroxisomes.
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pPhi-Yellow-peroxi
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FP606
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References:
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Gould SJ, Keller GA, Hosken N, Wilkinson J, Subramani S.
A conserved tripeptide sorts proteins to peroxisomes.
J Cell Biol. 1989; 108 (5):1657-64. / pmid: 2654139
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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