Phi-Yellow fluorescent proteins

- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines

Performance and use

Phi-Yellow proteins can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with PhiYFP and PhiYFP-m expression vectors produce bright fluorescence in 10-12 hours after transfection. No cytotoxic effects or visible protein aggregation are observed.

Fluorescent microscopy of transiently transfected mammalian cells expressing Phi-Yellow proteins.

Suitability of Phi-Yellow proteins to generate stably transfected cells has been proven by Marinpharm company.

Fluorescent microscopy of stably transfected mammalian cells expressing PhiYFP in cytosol. (A) M3 mouse melanoma; (B) T-406 human glioma; (C) PC-12 rat phaeochromocytoma cells; (D) PC-12 cells after the addition of nerve growth factor; (E) Walker 256 rat tumour cells; (F) BC3H1 cells; (G) T24 human bladder carcinoma cells; (H) T24 cells expressing destabilized variant PhiYFP-m-dest1. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).

Despite dimerization capacity, Phi-Yellow proteins demonstrate successful performance in fusions with subcellular localization signals and many cellular proteins. However, we recommend that you use TagFPs for protein labeling applications.
Important note: PhiYFP allows generation of fusions to its N-terminus, whereas PhiYFP-m is optimized to generate fusions to its C-terminus. PhiYFP can not be used to generate C-terminal fusions.

Phi-Yellow proteins can be used in multicolor labeling applications with blue, cyan, green, red, and far-red fluorescent dyes.

	Fluorescent microscopy of stably transfected mammalian cells expressing Phi-Yellow-tagged fusions. (A-B) Mitochondria-targeted PhiYFP in (A) 3T3 mouse fibroblasts; (B) PtK rat kangaroo cells; (C) T24 human bladder carcinoma cells expressing peroxisome-targeted PhiYFP-m; (D) PhiYFP-m fusion with β-actin in PtK rat kangaroo cells. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).