PhiYFP normalized excitation (thin line) and emission (thick line) spectra.
Download PhiYFP spectra (xls)
| | CHARACTERISTIC | PhiYFP | PhiYFP-m |
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 26.8 | 26.8 | | Polypeptide length, aa | 234 | 234 | | Fluorescence color | yellow | yellow | | Excitation maximum, nm | 525 | 525 | | Emission maximum, nm | 537 | 537 | | Quantum yield | 0.40 | 0.39 | | Extinction coefficient, M-1cm-1 | 130 000 | 124 000 | | Brightness* | 52.0 | 48.4 | | Brightness, % of EGFP | 158 | 147 | | pKa | 6.0 | 6.0 | | Structure | weak dimer | weak dimer | | Aggregation | no | no | | Maturation rate at 37°C | fast | fast | | Photostability | high | high | | Cell toxicity | not observed | not observed | | Main advantages | bright and fast-maturing yellow fluorescent proteins suitable for generation of stably transfected cell lines | | Possible limitations | weak dimers with limited applicability for fusions generation; PhiYFP can not be used for generation of fusions to its C-terminus |
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