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    PA-TagRFP

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Photoactivatable red fluorescent protein PA-TagRFP

- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for super-resolution imaging

PA-TagRFP is a photoactivatable mutant of the bright monomeric red fluorescent protein TagRFP [Subach et al., 2010]. PA-TagRFP is capable of irreversible photoconversion from non-fluorescent to red fluorescent form (with excitation/emission maxima at 562 nm and 595 nm, respectively) in response to UV-violet light irradiation.

High brightness, photostability and monomeric nature of PA-TagRFP make it an excellent protein tag for both conventional microscopy and super-resolution PALM imaging techniques [Subach et al., 2010].

detailed description

 
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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


PA-TagRFP expression/source vectors

pPA-TagRFP-C FP811 Mammalian expression vector encoding humanized PA-TagRFP and allowing its expression and generation of fusions to the PA-TagRFP C-terminus 20 μg € 400 / 200*
pPA-TagRFP-N FP812 Mammalian expression vector encoding humanized PA-TagRFP and allowing its expression and generation of fusions to the PA-TagRFP N-terminus 20 μg € 400 / 200*
pPA-TagRFP-tubulin FP814 Mammalian expression vector encoding humanized PA-TagRFP fused with human α-tubulin 20 μg € 400 / 200*
pPA-TagRFP-H2B FP815 Mammalian expression vector encoding humanized PA-TagRFP fused with human histone H2B 20 μg € 400 / 200*


Antibody against PA-TagRFP

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry 100 μg € 220

References:

  • Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. J Am Chem Soc. 2010; 132 (18):6481-91. doi: 10.1021/ja100906g / pmid: 20394363
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