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Red fluorescent protein FusionRed
- Superior performance in fusions
- Low cytotoxicity
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and long-term experiments
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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Humanized FusionRed
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FusionRed codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pFusionRed-N
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FP412
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FusionRed-Cx43 fusion
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Rat connexin 43 is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of connexin 43 in living cells.
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pFusionRed-Cx43
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FP417
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FusionRed-f-mem fusion
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20 amino acid farnesylation signal from c-Ha-Ras is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of plasma membrane.
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pFusionRed-f-mem
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FP418
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FusionRed-H2B fusion
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Human histone H2B is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of histone H2B in living cells.
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pFusionRed-H2B
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FP421
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FusionRed-endo fusion
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Human RhoB GTPase is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of vesicles of the endocytic pathway.
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pFusionRed-endo
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FP427
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FusionRed-PDHA1 fusion
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Human pyruvate dehydrogenase alpha 1 (PDHA1) is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of PDHA1 in living cells.
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pFusionRed-PDHA1
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FP430
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FusionRed-Rab5a fusion
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Human Ras-related protein Rab-5A is fused to the FusionRed C-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of Rab-5A in living cells.
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pFusionRed-Rab5a
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FP431
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FusionRed-talin fusion
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Mouse talin-1 protein is fused to the FusionRed N-terminus. When expressed in mammalian cells, this fusion provides red fluorescent labeling of talin in living cells.
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pFusionRed-talin
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FP432
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References:
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Fliegel L, Burns K, MacLennan DH, Reithmeier RA, Michalak M.
Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum.
J Biol Chem. 1989; 264 (36):21522-8. / pmid: 2600080
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Munro S, Pelham HR.
A C-terminal signal prevents secretion of luminal ER proteins.
Cell. 1987; 48 (5):899-907. / pmid: 3545499
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Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D.
A monomeric red fluorescent protein with low cytotoxicity.
Nat Commun. 2012; 3 :1204. doi: 10.1038/ncomms2208 / pmid: 23149748
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