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    Case12

Calcium ion sensor Case12

- High dynamic range detection of intracellular Ca2+ level changes
- High selectivity and sensitivity, relatively high pH stability
- Fast maturation, high brightness of fluorescent response
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring changes in Ca2+ concentration inside living cells

Case12 is a high dynamic range genetically encoded fluorescent sensor for direct measurement of changes of intracellular Ca2+ under various physiological and pathological conditions [Souslova et al., 2007]. The sensor is sensitive to changes of calcium concentration in a physiological range from a hundred nanomoles to micromoles with a high signal-to-noise ratio. Binding of Ca2+ is fast and reversible, allowing monitoring of high-frequency Ca2+ oscillations. In response to Ca2+ concentration rise, Case12 shows up to 12-fold increase of fluorescence brightness. Fluorescence of Case12 is characterized by single excitation/emission maxima peaked at 491/516 nm.

Case12 is recommended for monitoring change of calcium concentration inside living cells during various physiological and pathological conditions.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Case12 expression/source vectors

pCase12-cyto FP991 Mammalian expression vector allowing Case12 expression in cytosol under the control of CMV promoter 20 μg € 400 / 200*
pCase12-mito FP992 Mammalian expression vector encoding mitochondria-targeted Case12 20 μg € 400 / 200*


Antibody against Case12

Anti-GFP
antibody
AB011 Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer 100 μg € 220
Anti-Tag(CGY)FP
antibody
AB121 Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, Case12, HyPer, EGFP, BrUSLEE and SLIM 100 μg € 220

References:

  • Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM. Single fluorescent protein-based Ca2+ sensors with increased dynamic range. BMC Biotechnol. 2007; 7 :37. / pmid: 17603870
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