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RESEARCH PRODUCTS/Genetically-encoded fluorescent sensors:

    Case12

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References citing Case12:
  • Shelukhina I, Spirova E, Kudryavtsev D, Ojomoko L, Werner M, Methfessel C, Hollmann M, Tsetlin V.
    Calcium imaging with genetically encoded sensor Case12: Facile analysis of α7/α9 nAChR mutants.
    PLoS One. 2017 Aug 10;12(8):e0181936 doi: 10.1371/journal.pone.0181936
    pmid: 28797116
     
  • Niescier RF, Chang KT, Min KT.
    Miro, MCU, and calcium: bridging our understanding of mitochondrial movement in axons.
    Front Cell Neurosci. 2013 Sep 10;7:148 doi: 10.3389/fncel.2013.00148
    pmid: 24058334
  1. Minireview
  • Shaw JL, Chang KT.
    Nebula/DSCR1 Upregulation Delays Neurodegeneration and Protects against APP-Induced Axonal Transport Defects by Restoring Calcineurin and GSK-3β Signaling.
    PLoS Genet. 2013 Sep;9(9):e1003792 doi: 10.1371/journal.pgen.1003792
    pmid: 24086147
  1. Constructs used: UAS-Case12 generated by inserting Case12 (from pCase12-mito vector) into pINDY6 vector.
  2. Expression system: Drosophila melanogaster flies.
  3. Detection system: Zeiss LSM5 confocal microscope.
  • Zhdanov AV, Waters AH, Golubeva AV, Dmitriev RI, Papkovsky DB.
    Availability of the key metabolic substrates dictates the respiratory response of cancer cells to the mitochondrial uncoupling.
    Biochim Biophys Acta. 2013 Jul 23;1837(1):51-62 doi: 10.1016/j.bbabio.2013.07.008
    pmid: 23891695
  1. Protonophore FCCP decreases mitochondrial Ca2+.
  2. Constructs used: pCase12-mito vector.
  3. Expression system: PC12 cell, transient expression.
  4. Detection system: Olympus FV1000 confocal laser scanning microscope. Case12 was excited at 488 nm (2.5–5% of maximal laser power) with emission collected at 500–540 nm.
  • Kasymov V, Larina O, Castaldo C, Marina N, Patrushev M, Kasparov S, Gourine AV.
    Differential sensitivity of brainstem versus cortical astrocytes to changes in pH reveals functional regional specialization of astroglia.
    J Neurosci. 2013 Jan 9;33(2):435-41 doi: 10.1523/JNEUROSCI.2813-12.2013
    pmid: 23303924
  1. Monitoring astrocyte activity by measuring intracellular Ca2+.
  2. Constructs used: Case12, subcloned to adenoviral vector AVV-sGFAP.
  3. Expression system: Rat primary astroglial cell cultures, adenoviral transduction.
  4. Detection system: Olympus I×71 inverted microscope.
  • Bonekamp NA, Islinger M, Lázaro MG, Schrader M.
    Cytochemical detection of peroxisomes and mitochondria.
    Methods Mol Biol. 2013;931:467-82 doi: 10.1007/978-1-62703-056-4_24
    pmid: 23027018
  1. Protocols for dual peroxisomes and mitochondria staining.
  • Housley GD.
    Recent insights into the regulation of breathing.
    Auton Neurosci. 2011 Oct 28;164(1-2):3-5 doi: 10.1016/j.autneu.2011.08.002
    pmid: 21852203
  1. Minireview
  • Wang X, Su B, Liu W, He X, Gao Y, Castellani RJ, Perry G, Smith MA, Zhu X.
    DLP1-dependent mitochondrial fragmentation mediates 1-methyl-4-phenylpyridinium toxicity in neurons: implications for Parkinson's disease.
    Aging Cell. 2011 Oct;10(5):807-23 doi: 10.1111/j.1474-9726.2011.00721.x
    pmid: 21615675
  1. An environmental toxin MPP+ increases intracellular Ca2+ concentration in neuroblastoma cells.
  2. Constructs used: pCase12-cyto vector.
  3. Expression system: Human SH-SY5Y neuroblastoma cells, transient expression.
  4. Detection system: Leica DMI6000 fluorescence microscope.
  • Chang KT, Niescier RF, Min KT.
    Mitochondrial matrix Ca2+ as an intrinsic signal regulating mitochondrial motility in axons.
    Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15456-61 doi: 10.1073/pnas.1106862108
    pmid: 21876166
  1. Constructs used: pCase12-cyto vector.
  2. Expression system: Mouse hippocampal neurons.
  3. Detection system: Leica SP5 scanning confocal microscope.
  • Figueiredo M, Lane S, Tang F, Liu BH, Hewinson J, Marina N, Kasymov V, Souslova EA, Chudakov DM, Gourine AV, Teschemacher AG, Kasparov S.
    Optogenetic experimentation on astrocytes.
    Exp Physiol. 2011 Jan;96(1):40-50 doi: 10.1113/expphysiol.2010.052597
    pmid: 21041318
  1. Review
  • Guo F, Liu B, Tang F, Lane S, Souslova EA, Chudakov DM, Paton JF, Kasparov S.
    Astroglia are a possible cellular substrate of angiotensin(1-7) effects in the rostral ventrolateral medulla.
    Cardiovasc Res. 2010 Aug 1;87(3):578-84 doi: 10.1093/cvr/cvq059
    pmid: 20200044
  1. Constructs used: adenoviral vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector).
  2. Expression system: primary cultures of rat neurones and astrocytes transfected with Case12 expressing adenoviral vectors.
  3. Detection system: Leica confocal SP1 scanner (excitation with 488nm laser line, minimum laser intensity).
  • Gourine AV, Kasymov V, Marina N, Tang F, Figueiredo MF, Lane S, Teschemacher AG, Spyer KM, Deisseroth K, Kasparov S.
    Astrocytes control breathing through pH-dependent release of ATP.
    Science. 2010 Jul 30;329(5991):571-5 doi: 10.1126/science.1190721
    pmid: 20647426
  1. Constructs used: adenoviral vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector).
  2. Expression system: primary cultures of rat astrocytes transfected with Case12 expressing adenoviral vector.
  3. Detection system: Leica fluorescence microscope and MiCam02 high-resolution camera (excitation with 488 nm laser line, at 0.5–0.7% of laser output, emission collected using a bandpass filter 505–550nm).
  • Zhu X, Caplan J, Mamillapalli P, Czymmek K, Dinesh-Kumar SP.
    Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death.
    EMBO J. 2010 Mar 3;29(5):1007-18 doi: 10.1038/emboj.2009.402
    pmid: 20075858
  1. Constructs used: plant expression vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector).
  2. Expression system: Case12 transiently expressed in N. benthamiana leaves using agroinfiltration.
  3. Detection system: Zeiss Axiovert 200M light microscope equipped with a LSM 510 NLO confocal microscope.
  • Hynes J, O'Riordan TC, Zhdanov AV, Uray G, Will Y, Papkovsky DB.
    In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assay.
    Anal Biochem. 2009 Jul 1;390(1):21-8 doi: 10.1016/j.ab.2009.04.016
    pmid: 19379702
  1. Constructs used: pCase12-mito vector.
  2. Expression system: Transiently transfected PC12 cells (rat pheochromocytoma).
  3. Detection system: Olympus FV 1000 confocal microscope (Case12 excitation at 488nm, 1.5% of laser power, emission detected at 500-550 nm).
  • Guo F, Souslova E, Chudakov D, Paton JFR, Kasparov S.
    Cellular substrates for angiotensin1-7 (Ang1-7) action in the rostral ventro-lateral medulla (RVLM) of the normotentsive and spontaneously hypertensive rat (SHR).
    FASEB J. 2009; 23(1_MeetingAbstracts):958.3
     
  • Souslova EA, Chudakov DM.
    Genetically encoded intracellular sensors based on fluorescent proteins.
    Biochemistry (Mosc). 2007 Jul;72(7):683-97
    pmid: 17680759
  1. Review
  • Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM.
    Single fluorescent protein-based Ca2+ sensors with increased dynamic range.
    BMC Biotechnol. 2007 Jun 29;7:37
    pmid: 17603870
  1. Constructs used: Case12 expressing plasmid.
  2. Expression system: M21 (human melanoma) and HeLa cells; primary rat cortical neurons.
  3. Detection system: Zeiss LSM 510 confocal microscope (Case12 excitation with 488nm laser line, emission collected at 500-540 nm).

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