Katushka2S normalized excitation (thin line) and emission (thick line) spectra.
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Download Katushka2S spectra (xls)
| | CHARACTERISTIC | |
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 26 | | Polypeptide length, aa | 235 | | Fluorescence color | far-red | | Excitation maximum, nm | 588 | | Emission maximum, nm | 633 | | Quantum yield | 0.44 | | Extinction coefficient, M-1cm-1 | 67 000 | | Brightness* | 29.5 | | Brightness, % of EGFP | 89 | | pKa | 5.4 | | Structure | dimer | | Aggregation | no | | Maturation rate at 37°C | super fast | | Photostability,widefield (sec) | 72 | | Cell toxicity | not observed | | Main advantages | super bright and fast-maturing far-red fluorescent protein; high pH-stability | | Possible limitations | dimer, limited applicability for fusions generation, medium photostability |
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