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    peTurboGFP-dest1

peTurboGFP-dest1 vector

cat.# FP524

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
peTurboGFP-dest1FP52420 μg€ 320 / 160*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
ReporterTurboGFP
Reporter codon usagemammalian
Promoter for TurboGFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use TurboGFP expression in mammalian cells; generation of fusions to the TurboGFP-dest1 N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboGFP-dest1
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboGFP: 1372-1374
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 1390-1509
Stop codon: 1510-1512
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 1521-1555
HBB intron 2: 1556-2406
First 233 bp of HBB exon 3: 2407-2639
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2781-2786 & 2810-2815
mRNA 3' ends: 2819 & 2831
f1 single-strand DNA origin: 2878-3333
Bacterial promoter for expression of Kanr gene
-35 region: 3395-3400
-10 region: 3418-3423
Transcription start point: 3430
SV40 origin of replication: 3674-3809
SV40 early promoter
Enhancer (72-bp tandem repeats): 3507-3578 & 3579-3650
21-bp repeats: 3654-3674, 3675-3695 & 3697-3717
Early promoter element: 3730-3736
Major transcription start points: 3726, 3764, 3770 & 3775
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3858-3860
Stop codon: 4650-4652
G->A mutation to remove Pst I site: 4040
C->A (Arg to Ser) mutation to remove BssH II site: 4386
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4888-4893 & 4901-4906
pUC plasmid replication origin: 5237-5880


Notice to Purchaser:

TurboGFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,678,893; European Pat. 1576157; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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