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pTagRFP-tubulin vector

cat.# FP145

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pTagRFP-tubulinFP14520 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for TagRFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red (orange) fluorescent labeling of α-tubulin filaments

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 2695-2697
Last amino acid in TagRFP: 1321-1323
Tubulin: 1342-2697
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2858-2863 & 2887-2892
mRNA 3' ends: 2896 & 2908
f1 single-strand DNA origin: 2955-3410
Bacterial promoter for expression of Kanr gene
-35 region: 3472-3477
-10 region: 3495-3500
Transcription start point: 3507
SV40 origin of replication: 3751-3886
SV40 early promoter
Enhancer (72-bp tandem repeats): 3584-3655 & 3656-3727
21-bp repeats: 3731-3751, 3752-3772 & 3774-3794
Early promoter element: 3807-3813
Major transcription start points: 3803, 3841, 3847 & 3852
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3935-3937
Stop codon: 4727-4729
G->A mutation to remove Pst I site: 4117
C->A (Arg to Ser) mutation to remove BssH II site: 4463
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4965-4970 & 4978-4983
pUC plasmid replication origin: 5314-5957

Notice to Purchaser:

TagRFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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