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pTagRFP-Golgi vector

cat.# FP367

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pTagRFP-GolgiFP36720 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for TagRFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red (orange) fluorescent labeling of Golgi apparatus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Golgi targeting sequence (GTS), fragment of human beta 1,4- galactosyltransferase: 597-842
TagRFP: 864-1577
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1730-1735 & 1759-1764
mRNA 3' ends: 1768 & 1780
f1 single-strand DNA origin: 1827-2282
Bacterial promoter for expression of Kanr gene
-35 region: 2344-2349
-10 region: 2367-2372
Transcription start point: 2379
SV40 origin of replication: 2623-2758
SV40 early promoter
Enhancer (72-bp tandem repeats): 2456-2527 & 2528-2599
21-bp repeats: 2603-2623, 2624-2644 & 2646-2666
Early promoter element: 2679-2685
Major transcription start points: 2675, 2713, 2719 & 2724
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2807-2809
Stop codon: 3599-3601
G->A mutation to remove Pst I site: 2989
C->A (Arg to Ser) mutation to remove BssH II site: 3335
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3837-3842 & 3850-3855
pUC plasmid replication origin: 4186-4829

Notice to Purchaser:

TagRFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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