pTagRFP-Golgi vector

cat.# FP367

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagRFP-Golgi FP367 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagRFP Reporter codon usage mammalian Promoter for TagRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red (orange) fluorescent labeling of Golgi apparatus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Golgi targeting sequence (GTS), fragment of human beta 1,4- galactosyltransferase: 597-842
TagRFP: 864-1577
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1730-1735 & 1759-1764
mRNA 3' ends: 1768 & 1780
f1 single-strand DNA origin: 1827-2282
Bacterial promoter for expression of Kan^r gene
-35 region: 2344-2349
-10 region: 2367-2372
Transcription start point: 2379
SV40 origin of replication: 2623-2758
SV40 early promoter
Enhancer (72-bp tandem repeats): 2456-2527 & 2528-2599
21-bp repeats: 2603-2623, 2624-2644 & 2646-2666
Early promoter element: 2679-2685
Major transcription start points: 2675, 2713, 2719 & 2724
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2807-2809
Stop codon: 3599-3601
G->A mutation to remove Pst I site: 2989
C->A (Arg to Ser) mutation to remove BssH II site: 3335
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3837-3842 & 3850-3855
pUC plasmid replication origin: 4186-4829