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pTagCFP-mito vector

cat.# FP117

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pTagCFP-mitoFP11720 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for TagCFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use cyan fluorescent labeling of mitochondria

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagCFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-704
Start of TagCFP coding sequence (ATG): 705-707
Stop codon: 1419-1421
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1575-1580 & 1604-1609
mRNA 3' ends: 1613 & 1625
f1 single-strand DNA origin: 1672-2127
Bacterial promoter for expression of Kanr gene
-35 region: 2189-2194
-10 region: 2212-2217
Transcription start point: 2224
SV40 origin of replication: 2468-2603
SV40 early promoter
Enhancer (72-bp tandem repeats): 2301-2372 & 2373-2444
21-bp repeats: 2448-2468, 2469-2489 & 2491-2511
Early promoter element: 2524-2530
Major transcription start points: 2520, 2558, 2564 & 2569
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2652-2654
Stop codon: 3444-3446
G->A mutation to remove Pst I site: 2834
C->A (Arg to Ser) mutation to remove BssH II site: 3180
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3682-3687 & 3695-3700
pUC plasmid replication origin: 4031-4674

Notice to Purchaser:

TagCFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 06809023 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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