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pTagBFP-tubulin vector

cat.# FP175

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pTagBFP-tubulinFP17520 μg€ 400 / 200*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for TagBFPPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use blue fluorescent labeling of α-tubulin filaments

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609
Stop codon: 2677-2679
Last amino acid in TagRFP: 1303-1305
Tubulin: 1324-2679
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2840-2845 & 2869-2874
mRNA 3' ends: 2878 & 2890
f1 single-strand DNA origin: 2937-3392
Bacterial promoter for expression of Kanr gene
-35 region: 3454-3459
-10 region: 3477-3482
Transcription start point: 3489
SV40 origin of replication: 3733-3868
SV40 early promoter
Enhancer (72-bp tandem repeats): 3566-3637 & 3638-3709
21-bp repeats: 3713-3733, 3734-3754 & 3756-3776
Early promoter element: 3789-3795
Major transcription start points: 3785, 3823, 3829 & 3834
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3917-3919
Stop codon: 4709-4711
G->A mutation to remove Pst I site: 4099
C->A (Arg to Ser) mutation to remove BssH II site: 4445
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4947-4952 & 4960-4965
pUC plasmid replication origin: 5296-5939

Notice to Purchaser:

TagBFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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