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pFusionRed-ER vector

cat.# FP420

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pFusionRed-ERFP42020 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of the lumen of the endoplasmic reticulum

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
FusionRed-ER fusion: 603-1397
Start codon (ATG): 603-605
Calreticulin signal sequence: 603-653
FusionRed: 669-1364
ER retention sequence (KDEL): 1383-1394
Stop codon: 1395-1397
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1589-1594 & 1618-1623
mRNA 3' ends: 1627 & 1639
f1 single-strand DNA origin: 1686-2141
Bacterial promoter for expression of Kanr gene
-35 region: 2203-2208
-10 region: 2226-2231
Transcription start point: 2238
SV40 origin of replication: 2482-2617
SV40 early promoter
Enhancer (72-bp tandem repeats): 2315-2386 & 2387-2458
21-bp repeats: 2462-2482, 2483-2503 & 2505-2525
Early promoter element: 2538-2544
Major transcription start points: 2534, 2572, 2578 & 2583
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2666-2668
Stop codon: 3458-3460
G->A mutation to remove Pst I site: 2848
C->A (Arg to Ser) mutation to remove BssH II site: 3194
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3696-3701 & 3709-3714
pUC plasmid replication origin: 4045-4688

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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