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    pFusionRed-CDC42

pFusionRed-CDC42 vector

cat.# FP435

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.


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vector information:
ProductCat.#SizePrice
pFusionRed-CDC42FP43520 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
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Vector typemammalian expression vector
ReporterFusionRed
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of CDC42

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
FusionRed
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
Human CDC42: 1351-1926
Stop codon: 1924-1926
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2087-2092 & 2116-2121
mRNA 3' ends: 2125 & 2137
f1 single-strand DNA origin: 2184-2639
Bacterial promoter for expression of Kanr gene
-35 region: 2701-2706
-10 region: 2724-2729
Transcription start point: 2736
SV40 origin of replication: 2980-3115
SV40 early promoter
Enhancer (72-bp tandem repeats): 2813-2884 & 2885-2956
21-bp repeats: 2960-2980, 2981-3001 & 3003-3023
Early promoter element: 3036-3042
Major transcription start points: 3032, 3070, 3076 & 3081
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3164-3166
Stop codon: 3956-3958
G->A mutation to remove Pst I site: 3346
C->A (Arg to Ser) mutation to remove BssH II site: 3692
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4194-4199 & 4207-4212
pUC plasmid replication origin: 4543-5186


Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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