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pFusionRed-CD151 vector

cat.# FP415

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

vector information:
pFusionRed-CD151FP41520 μg€ 480 / 240*
*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
Reporter codon usagemammalian
Promoter for FusionRedPCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use red fluorescent labeling of CD151

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
FusionRed-CD151 fusion: 613-2103
FusionRed: 613-1308
Start codon (ATG): 613-615
Last amino acid in FusionRed: 1306-1308
CD151: 1342-2103
Stop codon: 2101-2103
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2295-2300 & 2324-2329
mRNA 3' ends: 2333 & 2345
f1 single-strand DNA origin: 2392-2847
Bacterial promoter for expression of Kanr gene
-35 region: 2909-2914
-10 region: 2932-2937
Transcription start point: 2944
SV40 origin of replication: 3188-3323
SV40 early promoter
Enhancer (72-bp tandem repeats): 3021-3092 & 3093-3164
21-bp repeats: 3168-3188, 3189-3209 & 3211-3231
Early promoter element: 3244-3250
Major transcription start points: 3240, 3278, 3284 & 3289
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3372-3374
Stop codon: 4164-4166
G->A mutation to remove Pst I site: 3554
C->A (Arg to Ser) mutation to remove BssH II site: 3900
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4402-4407 & 4415-4420
pUC plasmid replication origin: 4751-5394

Notice to Purchaser:

FusionRed-related materials (also referred to as "Products") are intended for research use only. The Products are covered by European Pat. 1994149 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

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