pCase12-mito vector

cat.# FP992

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pCase12-mito FP992 20 μg € 400 / 200* *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer. The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. The product is not available in certain countries. Please contact Evrogen or your local distributor before ordering. Vector type mammalian expression vector Reporter Case12 Reporter codon usage mammalian Promoter for Case12 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of mitochondria-targeted fluorescent Ca2+ sensor Case12 in mammalian cells under the control of CMV promoter; source of mitochondria-targeted Case12 coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Case12-Mito fusion
Start codon (ATG): 597-599
Mitochondrial localization signal (MLS): 597-683
Start of Case12 coding sequence (ATG): 705-707
Stop codon: 1950-1952
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1948-1953, 2106-2111 & 2135-2140
mRNA 3' ends: 2144 & 2156
f1 single-strand DNA origin: 2203-2658
Bacterial promoter for expression of Kan^r gene
-35 region: 2720-2725
-10 region: 2743-2748
Transcription start point: 2755
SV40 origin of replication: 2999-3134
SV40 early promoter
Enhancer (72-bp tandem repeats): 2832-2903 & 2904-2975
21-bp repeats: 2979-2999, 3000-3020 & 3022-3042
Early promoter element: 3055-3061
Major transcription start points: 3051, 3089, 3095 & 3100
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3183-3185
Stop codon: 3975-3977
G->A mutation to remove Pst I site: 3365
C->A (Arg to Ser) mutation to remove BssH II site: 3711
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4213-4218 & 4226-4231
pUC plasmid replication origin: 4562-5205