TurboFP635 normalized excitation (thin line) and emission (thick line) spectra.
Spectra viewer tool Download TurboFP635 spectra (xls)
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| | Molecular weight, kDa | 26 | | Polypeptide length, aa | 235 | | Fluorescence color | far-red | | Excitation maximum, nm | 588 | | Emission maximum, nm | 635 | | Quantum yield | 0.34 | | Extinction coefficient, M-1cm-1 | 65 000 | | Brightness* | 22.1 | | Brightness, % of EGFP | 67 | | pKa | 5.5 | | Structure | dimer | | Aggregation | no | | Maturation rate at 37°C | super fast | | Photostability | high | | Cell toxicity | not observed | | Main advantages | bright and fast-maturing far-red fluorescent protein; high pH-stability and photostability | | Possible limitations | dimer, limited applicability for fusions generation |
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