TagGFP2 normalized excitation (thin line) and emission (thick line) spectra.
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| CHARACTERISTIC | |
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| Molecular weight, kDa | 27 | Polypeptide length, aa | 238 | Fluorescence color | green | Excitation maximum, nm | 483 | Emission maximum, nm | 506 | Quantum yield | 0.6 | Extinction coefficient, M-1cm-1 | 56 500 | Brightness* | 33.9 | Brightness, % of EGFP | 105 | Fluorescence lifetime, ps | 3200 | Fluorescence intensity decay | nearly single-exponential | pKa | 5.0 | Structure | monomer | Aggregation | no | Maturation rate at 37°C | fast | Photostability | high | Cell toxicity | not observed | Main advantages | bright green monomeric fluorescent protein |
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| Maturation curves for TagGFP2 and parental TagGFP.
Dashed lines indicate maturation half-times of 11 min and 18 min for TagGFP2 (dark green curve) and TagGFP (light green curve), respectively. Recording of protein maturation was started when about 7% from their maximal fluorescence has been detected. Time point "0" was defined using an approximation of the beginning of the maturation curves with straight lines.
Data from Subach et al., 2008.
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