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    TagGFP2

Green fluorescent protein TagGFP2

- Bright green fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling and FRET applications

Main properties

TagGFP2 spectra

TagGFP2 normalized excitation (thin line) and emission (thick line) spectra.

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Download TagGFP2 spectra (xls)

CHARACTERISTIC
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Molecular weight, kDa27
Polypeptide length, aa238
Fluorescence colorgreen
Excitation maximum, nm483
Emission maximum, nm506
Quantum yield0.6
Extinction coefficient, M-1cm-156 500
Brightness*33.9
Brightness, % of EGFP105
Fluorescence lifetime, ps3200
Fluorescence intensity decaynearly single-exponential
pKa5.0
Structuremonomer
Aggregationno
Maturation rate at 37°Cfast
Photostabilityhigh
Cell toxicitynot observed
Main advantagesbright green monomeric fluorescent protein

Maturation curves for TagGFP2 and parental TagGFP.

Dashed lines indicate maturation half-times of 11 min and 18 min for TagGFP2 (dark green curve) and TagGFP (light green curve), respectively. Recording of protein maturation was started when about 7% from their maximal fluorescence has been detected. Time point "0" was defined using an approximation of the beginning of the maturation curves with straight lines.

Data from Subach et al., 2008.

References:

  • Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV. Conversion of Red Fluorescent Protein into a Bright Blue Probe. Chem Biol. 2008; 15 (10):1116-24. doi: 10.1016/j.chembiol.2008.08.006 / pmid: 18940671
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